Human Cytoglobin ELISA (ab277460)
Key features and details
- Sensitivity: 0.3 ng/ml
- Range: 0.287 ng/ml - 70 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Cytoglobin ELISA -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.3 ng/ml -
Range
0.287 ng/ml - 70 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 90.59 82% - 101% Plasma 87.98 84% - 94% Cell culture media 74.86 69% - 87% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Cytoglobin ELISA Kit (ab277460) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Cytoglobin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Cytoglobin coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Cytoglobin present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Cytoglobin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Cytoglobin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl Biotinylated Anti-Human Cytoglobin Antibody 2 vials Anti-Human Cytoglobin coated Microplate 1 unit Human Cytoglobin standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
May have a protective function during conditions of oxidative stress. May be involved in intracellular oxygen storage or transfer. -
Tissue specificity
Ubiquitously expressed. Highest expression in heart, stomach, bladder and small intestine. -
Sequence similarities
Belongs to the globin family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- Cygb
- CYGB_HUMAN
- Cytoglobin
see all -
Database links
- Entrez Gene: 114757 Human
- Omim: 608759 Human
- SwissProt: Q8WWM9 Human
- Unigene: 95120 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.