Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ZPI antibody [EPR23683-118] - BSA and Azide free (ab273634)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23683-118] to ZPI - BSA and Azide free
  • Suitable for: IHC-P, IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ZPI antibody [EPR23683-118] - BSA and Azide free
    See all ZPI primary antibodies

  • Description

    Rabbit monoclonal [EPR23683-118] to ZPI - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IP, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-Fr

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human, mouse and rat plasma. Human liver cancer tissue lysate. Human serum. IHC-P: Human liver, pancreas and placenta tissue; Rat liver tissue. IP: Human plasma.

  • General notes

    ab273634 is the carrier-free version of ab273627. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab273634 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZPI antibody [EPR23683-118] - BSA and Azide free (ab273634)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZPI antibody [EPR23683-118] - BSA and Azide free (ab273634)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling ZPI with ab273627 at 1/1000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human liver (PMID: 20024489). The section was incubated with ab273627 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273627).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZPI antibody [EPR23683-118] - BSA and Azide free (ab273634)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZPI antibody [EPR23683-118] - BSA and Azide free (ab273634)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling ZPI with ab273627 at 1/1000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on pancreas islets (PMID: 16728581). The section was incubated with ab273627 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273627).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZPI antibody [EPR23683-118] - BSA and Azide free (ab273634)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZPI antibody [EPR23683-118] - BSA and Azide free (ab273634)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling ZPI with ab273627 at 1/1000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on plasma in human placenta. The section was incubated with ab273627 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273627).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZPI antibody [EPR23683-118] - BSA and Azide free (ab273634)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZPI antibody [EPR23683-118] - BSA and Azide free (ab273634)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling ZPI with ab273627 at 1/1000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat liver (PMID: 20024489). The section was incubated with ab273627 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273627).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZPI antibody [EPR23683-118] - BSA and Azide free (ab273634)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZPI antibody [EPR23683-118] - BSA and Azide free (ab273634)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling ZPI with ab273627 at 1/1000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The section was incubated with ab273627 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Negative control: No staining on human cererbum (PMID: 20024489). 

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273627).

  • Immunoprecipitation - Anti-ZPI antibody [EPR23683-118] - BSA and Azide free (ab273634)
    Immunoprecipitation - Anti-ZPI antibody [EPR23683-118] - BSA and Azide free (ab273634)

    ZPI was immunoprecipitated from 0.35 mg human plasma with ab273627 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273627 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Human plasma lysate 10µg

    Lane 2: ab273627 IP in human plasma 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab273627 in human plasma 

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    The expression profile observed is consistent with what has been described in the literature (PMID:10460162).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273627).

  • Anti-ZPI antibody [EPR23683-118] - BSA and Azide free (ab273634)
    Anti-ZPI antibody [EPR23683-118] - BSA and Azide free (ab273634)

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