Cow Transferrin ELISA Kit (ab190519)
Key features and details
- Sensitivity: 7.449 ng/ml
- Range: 18.75 ng/ml - 600 ng/ml
- Sample type: Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Cow
Overview
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Product name
Cow Transferrin ELISA Kit
See all Transferrin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum Inter-assay Sample n Mean SD CV% Serum -
Sample type
Serum, Plasma, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
7.449 ng/ml -
Range
18.75 ng/ml - 600 ng/ml -
Recovery
> 85 %
Sample specific recovery Sample type Average % Range Serum > 85 18.75ng/ml - 600ng/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Cow -
Product overview
Abcam’s Transferrin Cow ELISA Kit (ab190519) is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Transferrin in Cow serum, plasma and tissue extracts.
In this assay the Transferrin present in samples reacts with the anti-Transferrin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Transferrin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Transferrin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Transferrin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Transferrin in the test sample. The quantity of Transferrin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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Notes
Transferrin is a metal-combining protein that reversibly bound to acid-soluble iron in plasma. It functions to transport iron to the bone marrow and to tissue storage organs such as the liver. Transferrin also participates in the regulation and control of iron absorption and protects against iron intoxication. Like haptoglobin, the carrier of hemoglobin, transferrin is synthesized in the liver, but unlike haptoglobin transferrin is returned to the circulation after unloading its iron in the reticuloendothelial system.
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Platform
Microplate
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 20X Diluent Concentrate 1 x 50ml 20X Wash Buffer Concentrate 1 x 50ml Stop Solution 1 x 12ml Chromogen Substrate Solution 1 x 12ml Transferrin Cow Antibody coated microwells 1 x 96 tests Transferrin Cow Calibrator (lyophilized) 1 vial Transferrin Cow HRP Conjugate 1 vial -
Research areas
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Function
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Involvement in disease
Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. -
Sequence similarities
Belongs to the transferrin family.
Contains 2 transferrin-like domains. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Apotransferrin
- Beta 1 metal binding globulin
- Beta-1 metal-binding globulin
see all -
Database links
- Entrez Gene: 280705 Cow
- SwissProt: Q29443 Cow
Images
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Standard Curve
Representative Standard Curve using ab190519 (Transferrin Cow ELISA Kit).