Iron Stain Kit (Prussian Blue Stain) (ab150674)
Overview
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Product name
Iron Stain Kit (Prussian Blue Stain)
See all Iron kits -
Product overview
Iron Stain Kit (Prussian Blue stain) ab150674 is intended for use in the detection of ferric iron in tissues, blood smears, or bone marrow smears. Ferric iron is normally found in small amounts in bone marrow and the spleen. Abnormally large deposits may be seen in hemochromatosis and hemosiderosis.
This iron stain kit is based on the Prussian Blue stain reaction in which ionic iron reacts with acid ferrocyanide producing a blue color.
Iron stain / Prussian Blue stain protocol summary:
- deparaffinize sections if necessary and rehydrate
- incubate for 3 min in iron stain (mix of potassium ferrocyanide and hydrochloric acid)
- rinse in water
- counterstain in nuclear fast red
- rinse in water
- dehydrate in alcohol and mountOther products for staining tissue sections
Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
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Notes
Staining Interpretation
Iron Bright Blue Nuclei Red Background Pink Control Tissue: Spleen, Bone Marrow
Properties
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Storage instructions
Store at room temperature. Please refer to protocols. -
Components 100 tests Hydrochloric Acid Solution (2%) 1 x 500ml Nuclear Fast Red Solution (Enhanced) 1 x 125ml Potassium ferrocyanide solution 1 x 500ml -
Research areas
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Relevance
Iron is essential to nearly all known organisms. It is generally stored in the centre of metalloproteins, in the heme complex, and in oxygen carrier proteins. Inorganic iron also contributes to redox reactions in the iron-sulfur clusters of many enzymes, such as nitrogenase and hydrogenase. -
Alternative names
- Fe
- Fe++
- Fe+++
Images
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ab150674 - Iron Stain Kit
ab150674 Iron Stain Kit staining formalin-fixed-paraffin embedded human liver.
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ab150674 - Iron Stain Kitab150674 (Iron stain) staining iron deposits (blue) in formalin fixed paraffin embedded normal human liver, counterstained with nuclear fast red (pink).
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
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ab150674 - Iron Stain KitStaining of Human Kidney using ab150674 - Iron Stain Kit.