Anti-Hepcidin-25 antibody (ab30760)
Key features and details
- Rabbit polyclonal to Hepcidin-25
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Hepcidin-25 antibody
See all Hepcidin-25 primary antibodies -
Description
Rabbit polyclonal to Hepcidin-25 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human Hepcidin-25.
Read Abcam's proprietary immunogen policy (Peptide available as ab31875.) -
Positive control
- WB: Recombinant Hepcidin protein. IHC-P: Human liver tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab30760 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB 1/10 - 1/100. Detects a band of approximately 32 kDa (predicted molecular weight: 3 kDa). IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Target
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Function
Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes (PubMed:22306005).
Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa (PubMed:11113131, PubMed:11034317). -
Tissue specificity
Highest expression in liver and to a lesser extent in heart and brain. Low levels in lung, tonsils, salivary gland, trachea, prostate gland, adrenal gland and thyroid gland. Secreted into the urine. -
Involvement in disease
Hemochromatosis 2B -
Sequence similarities
Belongs to the hepcidin family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 57817 Human
- Entrez Gene: 84506 Mouse
- Entrez Gene: 84604 Rat
- Omim: 606464 Human
- SwissProt: P81172 Human
- SwissProt: Q9EQ21 Mouse
- SwissProt: Q99MH3 Rat
- Unigene: 8821 Human
see all -
Form
Hepcidin produces a propeptide of 84 amino acids that undergoes enzymatic cleavage into mature peptides of 20, 22, and 25 amino acids. Active peptides are rich in cysteines that form intramolecular bonds and stabilize the beta-sheet structure -
Alternative names
- Hamp antibody
- HEPC antibody
- HEPC_HUMAN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin-25 antibody (ab30760)
IHC image of Hepcidin-25 staining in Human normal liver formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab30760, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Western blot - Anti-Hepcidin-25 antibody (ab30760)Anti-Hepcidin-25 antibody (ab30760) at 1 µg/ml + HAMP Human Full-length Recombinant Protein (Tagged) at 0.1 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 3 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
ab30760 was tested against Human HAMP Full-length Recombinant Protein (Tagged) predicted to run at 32kDa. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin-25 antibody (ab30760)This image is courtesy of Ruma Raha-Chowdhury (Cambridge University)Hepcidin-25 expression in rat liver cells using Rabbit polyclonal to Hepcidin-25 (ab30760). Immunohistochemistry was performed on PFA fixed cells, ab30760 was used at 1/1000 incubated overnight at RT. Haemotoxylin is used as a nuclear counterstain in this image.
Protocols
References (20)
ab30760 has been referenced in 20 publications.
- Zuo E et al. Increased expression of hepcidin and associated upregulation of JAK/STAT3 signaling in human gastric cancer. Oncol Lett 15:2236-2244 (2018). IHC-P . PubMed: 29434930
- van Swelm RPL et al. Endogenous hepcidin synthesis protects the distal nephron against hemin and hemoglobin mediated necroptosis. Cell Death Dis 9:550 (2018). PubMed: 29749404
- Jang M et al. Study of melatonin-mediated effects on various hepatic inflammatory responses stimulated by IL-6 in a new HepG2-on-a-chip platform. Biomed Microdevices 20:54 (2018). PubMed: 29946898
- Blanchette-Farra N et al. Contribution of three-dimensional architecture and tumor-associated fibroblasts to hepcidin regulation in breast cancer. Oncogene 37:4013-4032 (2018). PubMed: 29695834
- Liu J et al. Studying hepcidin and related pathways in osteoblasts using a mouse model with insulin receptor substrate 1-loss of function. Mol Med Rep 17:350-357 (2018). WB, IHC-P ; Mouse . PubMed: 29115497
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin-25 antibody (ab30760)
IHC image of Hepcidin-25 staining in Human normal liver formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab30760, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
-
Western blot - Anti-Hepcidin-25 antibody (ab30760)Anti-Hepcidin-25 antibody (ab30760) at 1 µg/ml + HAMP Human Full-length Recombinant Protein (Tagged) at 0.1 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 3 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
ab30760 was tested against Human HAMP Full-length Recombinant Protein (Tagged) predicted to run at 32kDa. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin-25 antibody (ab30760) This image is courtesy of Ruma Raha-Chowdhury (Cambridge University)
Hepcidin-25 expression in rat liver cells using Rabbit polyclonal to Hepcidin-25 (ab30760). Immunohistochemistry was performed on PFA fixed cells, ab30760 was used at 1/1000 incubated overnight at RT. Haemotoxylin is used as a nuclear counterstain in this image.
