Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RBP4 antibody [EP3657] (ab109193)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP3657] to RBP4
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RBP4 antibody [EP3657]
    See all RBP4 primary antibodies

  • Description

    Rabbit monoclonal [EP3657] to RBP4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human plasma, HepG2 cell lysates, Human fetal kidney, Mouse serum and Rat serum. ICC/IF: HeLa cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-RBP4 antibody [EP3657] (ab109193)
    Western blot - Anti-RBP4 antibody [EP3657] (ab109193)
    All lanes : Anti-RBP4 antibody [EP3657] (ab109193) at 1/5000 dilution

    Lane 1 : HepG2 (human hepatocellular carcinoma) whole cell lysate
    Lane 2 : Human fetal kidney

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    Blocking and diluting buffer 5% NFDM/TBST
  • Western blot - Anti-RBP4 antibody [EP3657] (ab109193)
    Western blot - Anti-RBP4 antibody [EP3657] (ab109193)
    Anti-RBP4 antibody [EP3657] (ab109193) at 1/5000 dilution + Mouse serum at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    Blocking and diluting buffer 5% NFDM/TBST
  • Western blot - Anti-RBP4 antibody [EP3657] (ab109193)
    Western blot - Anti-RBP4 antibody [EP3657] (ab109193)
    Anti-RBP4 antibody [EP3657] (ab109193) at 1/5000 dilution + Rat serum at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    Blocking and diluting buffer 5% NFDM/TBST
  • Western blot - Anti-RBP4 antibody [EP3657] (ab109193)
    Western blot - Anti-RBP4 antibody [EP3657] (ab109193)
    All lanes : Anti-RBP4 antibody [EP3657] (ab109193) at 1/1000 dilution (un-purified)

    Lane 1 : Human plasma lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RBP4 antibody [EP3657] (ab109193)
    Immunocytochemistry/ Immunofluorescence - Anti-RBP4 antibody [EP3657] (ab109193)

    Immunocytochemistry/immunofluorescence staining of 100% methanol fixed; 0.1% triton X 100 permeabilized HeLa (human cervix adenocarcinoma) cells with purified ab109193 at dilution of 1/100. The secondary antibody used was Alexa Fluor® 488; goat anti-rabbit IgG (ab150077) at a dilution of 1/1000. Nucleus was counter-stained with DAPI (blue). ab7291, a mouse anti-tubulin antibody (1/1000) was used to stain tubulin along with ab150120 (AlexaFluor®594 goat anti-mouse secondary, 1/1000) shown in the top right hand panel. The negative controls are shown in the bottom middle and right hand panels- for negative control 1 rabbit primary antibody and anti-mouse secondary antibody (ab150120) was used. For negative control 2 mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077) was used.

  • Immunocytochemistry/ Immunofluorescence - Anti-RBP4 antibody [EP3657] (ab109193)
    Immunocytochemistry/ Immunofluorescence - Anti-RBP4 antibody [EP3657] (ab109193)

    Immunofluorescent analysis of HeLa cells labelling RBP4 with un-purified ab109193 at a dilution of 1/100.

  • Anti-RBP4 antibody [EP3657] (ab109193)
    Anti-RBP4 antibody [EP3657] (ab109193)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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