Human Retinol Binding Protein 4 ELISA Kit (ab196264)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.6 pg/ml
- Range: 15.6 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Milk, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Retinol Binding Protein 4 ELISA Kit
See all RBP4 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 5.1% Inter-assay Sample n Mean SD CV% Serum 3 8.9% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2.6 pg/ml -
Range
15.6 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Saliva 104 99% - 108% Milk 103 100% - 108% Urine 110 106% - 113% Serum 109 104% - 111% Cell culture media 101 99% - 102% Hep Plasma 103 91% - 110% EDTA Plasma 108 106% - 110% Cit plasma 109 106% - 114% serum free media 103 93% - 113% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Retinol Binding Protein 4 ELISA Kit (ab196264) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Retinol Binding Protein 4 protein in edta plasma, milk, saliva, serum, urine, cit plasma, hep plasma, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Retinol Binding Protein 4 with 2.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Retinol-binding protein 4 (RBP4) is a circulating protein that binds to and transports retinol (vitamin A) in serum. The RBP4-retinol complex further interacts with prealbumin (also known as transthyretin) which increases its half-life in circulation. RBP4 is produced by hepatocytes and adipocytes. Loss of functional RBP4 protein results in serum retinol deficiency which can impact vision.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human RBP-4 Capture Antibody 1 x 600µl 10X Human RBP-4 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human RBP-4 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 2 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli. -
Involvement in disease
Defects in RBP4 are a cause of retinol-binding protein deficiency (RBP deficiency) [MIM:180250]. This condition causes night vision problems. It produces a typical 'fundus xerophthalmicus', featuring a progressed atrophy of the retinal pigment epithelium. -
Sequence similarities
Belongs to the calycin superfamily. Lipocalin family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- OTTHUMP00000020114
- OTTHUMP00000020115
- OTTHUMP00000020116
see all -
Database links
- Entrez Gene: 5950 Human
- Omim: 180250 Human
- SwissProt: P02753 Human
- Unigene: 50223 Human
Images
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Other - Human Retinol Binding Protein 4 ELISA Kit (ab196264)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human RBP-4 Competitor standard curve comparisonStandard curve comparison between Human RBP4 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of human RBP4 standard curve in Sample Diluent NS.The RBP4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native RBP4 in human serum and plasma samples.The concentrations of RBP4 were measured in duplicates, interpolated from the RBP4 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:400,000, plasma (citrate) 1:200,000, plasma (EDTA) 1: 100,000, and plasma (heparin) 1:200,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RBP4 concentration was determined to be 22.2 µg/mL in serum, 21.9 µg/mL in plasma (citrate), 15.1 µg/mL in plasma (EDTA) and 52.9 µg/mL in plasma (heparin).
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. Interpolated concentrations of native RBP4 in human saliva, urine, milk, and cell culture supernatant samples.The concentrations of RBP4 were measured in duplicates, interpolated from the RBP4 standard curves and corrected for sample dilution. Undiluted samples are as follows: Â saliva 1:80, urine 1: 160, milk 1:1000, and HEPG2 cell culture supernatant 1:1000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RBP4 concentration was determined to be 16.2 ng/mL in saliva, 66.3 ng/mL in urine, 111 ng/mL in milk and 1071 ng/mL in HEPG2 cell culture supernatant.
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Serum from ten individual healthy human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RBP4 concentration was determined to be 44 µg/mL with a range of 28 – 67 µg/mL.
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Bovine serum was prepared at 1% and diluted 2- fold for 8 points.Bovine reactivity was seen at serum levels greater than 0.03%.
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Sandwich ELISA - Human Retinol Binding Protein 4 ELISA Kit (ab196264)To learn more about the advantages of recombinant antibodies see here.
