Anti-T7 tag® antibody (ab9138)
Key features and details
- Goat polyclonal to T7 tag®
- Suitable for: ELISA, IP, WB, ICC, ChIP
- Reacts with: Species independent
- Isotype: unknown
Overview
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Product name
Anti-T7 tag® antibody
See all T7 tag® primary antibodies -
Description
Goat polyclonal to T7 tag® -
Host species
Goat -
Specificity
This antibody was verified by ELISA against peptide conjugated to BSA(MASMTGGQQMG/BSA). Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG. -
Tested applications
Suitable for: ELISA, IP, WB, ICC, ChIPmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
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General notes
T7-TAG® is a trademark of EMD Biosciences, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was affinity purified using the peptide immobilized on solid support.. -
Clonality
Polyclonal -
Isotype
unknown -
Light chain type
unknown -
Research areas
Images
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ChIP - Anti-T7 tag® antibody (ab9138)A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a V5 or T7- tagged GAL4 DNA Binding Domain construct. 48 hours post transfection Chromatin was prepared according to the Abcam X-ChIP protocol. The ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. A non-specific antibody was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR (SYBR Green approach).
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Western blot - Anti-T7 tag® antibody (ab9138)All lanes : Anti-T7 tag® antibody (ab9138) at 1/25000 dilution
Lane 1 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.2 µg
Lane 2 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.1 µg
Lane 3 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.05 µg
