Anti-Hepcidin-25 antibody [EPR18074] - BSA and Azide free (ab238974)
![Anti-Hepcidin-25 antibody [EPR18074] - BSA and Azide free (ab238974)](https://www.abcam.com/ps/products/238/ab238974/Images/ab238974-317637-238974.jpg "Anti-Hepcidin-25 antibody [EPR18074] - BSA and Azide free (ab238974)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18074] to Hepcidin-25 - BSA and Azide free
- Suitable for: WB
- Reacts with: Rat, Human
Overview
-
Product name
Anti-Hepcidin-25 antibody [EPR18074] - BSA and Azide free
See all Hepcidin-25 primary antibodies -
Description
Rabbit monoclonal [EPR18074] to Hepcidin-25 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Recombinant fragment
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Human plasma, serum and liver lysate.
-
General notes
Ab238974 is the carrier-free version of ab187778. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab238974 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18074 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Hepcidin-25 antibody [EPR18074] - BSA and Azide free (ab238974)All lanes : Anti-Hepcidin-25 antibody [EPR18074] (ab187778) at 1/1000 dilution
Lane 1 : Human plasma
Lane 2 : Human serum
Lane 3 : Human liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP), preadsorbed at 1/50000 dilution
Predicted band size: 9 kDa
Observed band size: 3 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST.
Dilution buffer: 1% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187778).
-
![Anti-Hepcidin-25 antibody [EPR18074] - BSA and Azide free (ab238974)](https://www.abcam.com/ps/products/238/ab238974/Images/ab238974-2-benefits-of-recombinant-antibodies.png)
Anti-Hepcidin-25 antibody [EPR18074] - BSA and Azide free (ab238974)
