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Anti-Hepcidin-25 antibody [EPR18074] - BSA and Azide free (ab238974)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18074] to Hepcidin-25 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-Hepcidin-25 antibody [EPR18074] - BSA and Azide free
    See all Hepcidin-25 primary antibodies

  • Description

    Rabbit monoclonal [EPR18074] to Hepcidin-25 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human plasma, serum and liver lysate.

  • General notes

    Ab238974 is the carrier-free version of ab187778. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238974 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Hepcidin-25 antibody [EPR18074] - BSA and Azide free (ab238974)
    Western blot - Anti-Hepcidin-25 antibody [EPR18074] - BSA and Azide free (ab238974)
    All lanes : Anti-Hepcidin-25 antibody [EPR18074] (ab187778) at 1/1000 dilution

    Lane 1 : Human plasma
    Lane 2 : Human serum
    Lane 3 : Human liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP), preadsorbed at 1/50000 dilution

    Predicted band size: 9 kDa
    Observed band size: 3 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST.

    Dilution buffer: 1% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187778).

  • Anti-Hepcidin-25 antibody [EPR18074] - BSA and Azide free (ab238974)
    Anti-Hepcidin-25 antibody [EPR18074] - BSA and Azide free (ab238974)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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