Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FOXA1 antibody [EPR10881-14] - BSA and Azide free (ab249749)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10881-14] to FOXA1 - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-FOXA1 antibody [EPR10881-14] - BSA and Azide free
    See all FOXA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR10881-14] to FOXA1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
    Unsuitable for: IP or WB

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab249749 is the carrier-free version of ab173287. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249749 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] - BSA and Azide free (ab249749)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] - BSA and Azide free (ab249749)

    This data was developed using ab173287, the same antibody clone in a different buffer formulation.

    IHC-Paraffin-embedded sections of Human prostate hyperplasia. 1:1000 dilution of ab173287 used. Counter stain = Hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-FOXA1 antibody [EPR10881-14] - BSA and Azide free (ab249749)
    Immunocytochemistry - Anti-FOXA1 antibody [EPR10881-14] - BSA and Azide free (ab249749)

    This data was developed using ab173287, the same antibody clone in a different buffer formulation.

    Left image: Immunofluorescent staining of SW480 cells using ab173287(1:1000 dilution). 1:200 dilution of Secondary antibody = Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078). Right image: Immunofluorescent staining of SW480 cells with DAPI. Fixative = 4% paraformaldehyde.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] - BSA and Azide free (ab249749)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] - BSA and Azide free (ab249749)

    This data was developed using ab173287, the same antibody clone in a different buffer formulation.

    IHC-Paraffin-embedded sections of Mouse colon. 1:1000 dilution of ab173287 used. Counter stain = Hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-FOXA1 antibody [EPR10881-14] - BSA and Azide free (ab249749)
    Immunocytochemistry - Anti-FOXA1 antibody [EPR10881-14] - BSA and Azide free (ab249749)

    This data was developed using ab173287, the same antibody clone in a different buffer formulation.

    Left image: Immunofluorescent staining of HepG2 cells using ab173287. 1:200 dilution of Secondary antibody = Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077). Right image: Immunofluorescent staining of HepG2 cells with DAPI. Fixative = 4% paraformaldehyde.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] - BSA and Azide free (ab249749)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] - BSA and Azide free (ab249749)

    This data was developed using ab173287, the same antibody clone in a different buffer formulation.

    IHC-Paraffin-embedded sections of Human breast carcinoma. 1:1000 dilution of ab173287 used. Counter stain = hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] - BSA and Azide free (ab249749)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] - BSA and Azide free (ab249749)

    This data was developed using ab173287, the same antibody clone in a different buffer formulation.

    ab173287 showing -ve staining in Human brain tissue. 1:1000 dilution of ab. Paraffin-embedded sections. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-FOXA1 antibody [EPR10881-14] - BSA and Azide free (ab249749)
    Anti-FOXA1 antibody [EPR10881-14] - BSA and Azide free (ab249749)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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