Anti-FOXA1 antibody (ab23738)
Key features and details
- Rabbit polyclonal to FOXA1
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-FOXA1 antibody
See all FOXA1 primary antibodies -
Description
Rabbit polyclonal to FOXA1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
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Positive control
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab23738 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications RatXenopus laevisApplication Abreviews Notes WB (5) Use a concentration of 0.7 - 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 49 kDa).Notes WB
Use a concentration of 0.7 - 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 49 kDa).Application notesIs unsuitable for ICC/IF,IHC-FoFr or IHC-P.Target
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Function
Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis. -
Tissue specificity
Highly expressed in prostate and ESR1-positive breast tumors. Overexpressed in esophageal and lung adenocarcinomas. -
Sequence similarities
Contains 1 fork-head DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3169 Human
- Entrez Gene: 15375 Mouse
- Entrez Gene: 25098 Rat
- Omim: 602294 Human
- SwissProt: P55317 Human
- SwissProt: P35582 Mouse
- SwissProt: P23512 Rat
- Unigene: 163484 Human
see all -
Alternative names
- forkhead box A1 antibody
- Forkhead box protein A1 antibody
- FOX A1 antibody
see all
Images
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Western blot - Anti-FOXA1 antibody - ChIP Grade (ab23738)All lanes : Anti-FOXA1 antibody (ab23738) at 0.7 µg/ml
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : FOXA1 knockout HAP1 whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lane 4 : A549 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 49 kDaLanes 1 - 4: Merged signal (red and green). Green - ab23738 observed at 49 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab23738 was shown to recognize FOXA1 in wild-type HAP1 cells as signal was lost at the expected MW in FOXA1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FOXA1 knockout samples were subjected to SDS-PAGE. Ab23738 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 0.7 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-FOXA1 antibody - ChIP Grade (ab23738)Anti-FOXA1 antibody (ab23738) at 1 µg/ml + Human heart tissue lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Predicted band size: 49 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Additional bands at: 31 kDa (possible cleavage fragment), 31 kDa (possible cross reactivity) -
Western blot - Anti-FOXA1 antibody - ChIP Grade (ab23738) -
Western blot - Anti-FOXA1 antibody - ChIP Grade (ab23738)This image is a courtesy of Anonymous AbreviewAll lanes : Anti-FOXA1 antibody (ab23738) at 1/1000 dilution
Lane 1 : Lysate prepared from human LNCaP cells
Lane 2 : Lysate prepared from MCF7 cells
Lane 3 : Lysate prepared from human LNCaP cells with ethanol as vehicle control
Lane 4 : Lysate prepared from human LNCaP cells with 100 nM DHT
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated goat polyclonal to rabbit IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Exposure time: 2 seconds
Protocols
References (103)
ab23738 has been referenced in 103 publications.
- Yee CH et al. Maintenance of the bladder cancer precursor urothelial hyperplasia requires FOXA1 and persistent expression of oncogenic HRAS. Sci Rep 9:270 (2019). PubMed: 30670749
- Glont SE et al. Comprehensive Genomic Analysis Reveals that the Pioneering Function of FOXA1 Is Independent of Hormonal Signaling. Cell Rep 26:2558-2565.e3 (2019). PubMed: 30840881
- Ding L et al. Deletion of Cdkn1b in ACI rats leads to increased proliferation and pregnancy-associated changes in the mammary gland due to perturbed systemic endocrine environment. PLoS Genet 15:e1008002 (2019). PubMed: 30893315
- Liu DW et al. Silencing of long noncoding RNA PVT1 inhibits podocyte damage and apoptosis in diabetic nephropathy by upregulating FOXA1. Exp Mol Med 51:88 (2019). PubMed: 31371698
- Berger A et al. N-Myc-mediated epigenetic reprogramming drives lineage plasticity in advanced prostate cancer. J Clin Invest 130:3924-3940 (2019). PubMed: 31260412
Images
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Western blot - Anti-FOXA1 antibody - ChIP Grade (ab23738)All lanes : Anti-FOXA1 antibody (ab23738) at 0.7 µg/ml
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : FOXA1 knockout HAP1 whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lane 4 : A549 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 49 kDaLanes 1 - 4: Merged signal (red and green). Green - ab23738 observed at 49 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab23738 was shown to recognize FOXA1 in wild-type HAP1 cells as signal was lost at the expected MW in FOXA1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FOXA1 knockout samples were subjected to SDS-PAGE. Ab23738 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 0.7 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-FOXA1 antibody - ChIP Grade (ab23738)Anti-FOXA1 antibody (ab23738) at 1 µg/ml + Human heart tissue lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Predicted band size: 49 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Additional bands at: 31 kDa (possible cleavage fragment), 31 kDa (possible cross reactivity)
-
Western blot - Anti-FOXA1 antibody - ChIP Grade (ab23738)
-
Western blot - Anti-FOXA1 antibody - ChIP Grade (ab23738) This image is a courtesy of Anonymous AbreviewAll lanes : Anti-FOXA1 antibody (ab23738) at 1/1000 dilution
Lane 1 : Lysate prepared from human LNCaP cells
Lane 2 : Lysate prepared from MCF7 cells
Lane 3 : Lysate prepared from human LNCaP cells with ethanol as vehicle control
Lane 4 : Lysate prepared from human LNCaP cells with 100 nM DHT
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated goat polyclonal to rabbit IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Exposure time: 2 seconds
