Alexa Fluor® 647 Anti-FOXA1 antibody [EPR10881] (ab196908)
![Alexa Fluor® 647 Anti-FOXA1 antibody [EPR10881] (ab196908)](https://www.abcam.com/ps/products/196/ab196908/Images/ab196908-237109-anti-foxa1-antibody-epr10881-alexa-fluor-647-immunofluorescence.jpg "Alexa Fluor® 647 Anti-FOXA1 antibody [EPR10881] (ab196908)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EPR10881] to FOXA1
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Overview
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Product name
Alexa Fluor® 647 Anti-FOXA1 antibody [EPR10881]
See all FOXA1 primary antibodies -
Description
Alexa Fluor® 647 Rabbit monoclonal [EPR10881] to FOXA1 -
Host species
Rabbit -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Positive control
- ICC/IF: HepG2 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10881 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-FOXA1 antibody [EPR10881] (ab196908)
ab196908 staining FOXA1 in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab196908 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/167 dilution, overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
This product also gave a positive signal under the same testing conditions in HepG2 cells fixed with 100% methanol (5 min).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Alexa Fluor® 647 Anti-FOXA1 antibody [EPR10881] (ab196908)](https://www.abcam.com/ps/products/196/ab196908/Images/ab196908-2-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 647 Anti-FOXA1 antibody [EPR10881] (ab196908)
