Anti-FOXA1 antibody (ab5089)
Key features and details
- Goat polyclonal to FOXA1
- Suitable for: WB, ICC
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-FOXA1 antibody
See all FOXA1 primary antibodies -
Description
Goat polyclonal to FOXA1 -
Host species
Goat -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig, Xenopus laevis, Zebrafish
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Immunogen
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Positive control
- Purchase matching WB positive control:Recombinant Human FOXA1 protein
- WB: HepG2 and MCF7 nuclear cell lysates; Mouse liver lysate. ICC: MCF7 and U2OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry - Anti-FOXA1 antibody (ab5089)
Immunofluorescent analysis of paraformaldehyde-fixed, 0.15% TritonX-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling FOXA1 with ab5089 at 10 ug/ml (1h). Image showing nuclear staining in MCF7 cells. An Alexa Fluor 488® secondary antibody was used at 2 ug/ml. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue).
Secondary antibody only control: Unimmunized goat IgG (10 ug/ml) followed by an Alexa Fluor 488® secondary antibody at 2ug/ml.
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Western blot - Anti-FOXA1 antibody (ab5089)Lane 1 : Anti-FOXA1 antibody (ab5089) at 0.1 µg/ml
Lane 2 : Anti-FOXA1 antibody (ab5089) at 0.3 µg/ml
Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) nuclear cell lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) nuclear cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 49.1 kDaWestern blot analysis labeling FOXA1 with ab5089.
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Western blot - Anti-FOXA1 antibody (ab5089)Anti-FOXA1 antibody (ab5089) at 0.1 µg/ml + Mouse liver lysate at 35 µg
Predicted band size: 49.1 kDaWestern blot labeling FOXA1 with ab5089 at 0.1 ug/ml.
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Immunocytochemistry - Anti-FOXA1 antibody (ab5089)Immunofluorescent analysis of paraformaldehyde-fixed, 0.15% TritonX-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling FOXA1 with ab5089 at 10 ug/ml (1h). Image showing nuclear staining in U-2 OS cells. An Alexa Fluor 488® secondary antibody was used at 2 ug/ml. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue).
Secondary antibody only control: Unimmunized goat IgG (10 ug/ml) followed by an Alexa Fluor 488® secondary antibody at 2ug/ml.
