Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FOXA1 antibody [EPR10881-14] (ab173287)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10881-14] to FOXA1
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-FOXA1 antibody [EPR10881-14]
    See all FOXA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR10881-14] to FOXA1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human FOXA1 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P55317

  • Positive control

    • Human breast carcinoma and prostate tissues; HepG2 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] (ab173287)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] (ab173287)

    IHC-Paraffin-embedded sections of Human prostate hyperplasia. 1:1000 dilution of ab173287 used. Counter stain = Hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-FOXA1 antibody [EPR10881-14] (ab173287)
    Immunocytochemistry/ Immunofluorescence - Anti-FOXA1 antibody [EPR10881-14] (ab173287)

    Left image: Immunofluorescent staining of SW480 cells using ab173287(1:1000 dilution). 1:200 dilution of Secondary antibody = Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078). Right image: Immunofluorescent staining of SW480 cells with DAPI. Fixative = 4% paraformaldehyde.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] (ab173287)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] (ab173287)

    IHC-Paraffin-embedded sections of Mouse colon. 1:1000 dilution of ab173287 used. Counter stain = Hematoxylin.  

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-FOXA1 antibody [EPR10881-14] (ab173287)
    Immunocytochemistry/ Immunofluorescence - Anti-FOXA1 antibody [EPR10881-14] (ab173287)

    Left image: Immunofluorescent staining of HepG2 cells using ab173287. 1:200 dilution of Secondary antibody = Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077). Right image: Immunofluorescent staining of HepG2 cells with DAPI. Fixative = 4% paraformaldehyde.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] (ab173287)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] (ab173287)

    IHC-Paraffin-embedded sections of Human breast carcinoma. 1:1000 dilution of ab173287 used. Counter stain = hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] (ab173287)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [EPR10881-14] (ab173287)

    ab173287 showing -ve staining in Human brain tissue. 1:1000 dilution of ab. Paraffin-embedded sections.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-FOXA1 antibody [EPR10881-14] (ab173287)
    Anti-FOXA1 antibody [EPR10881-14] (ab173287)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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