Anti-FOXA1 antibody (ab55178)
Key features and details
- Mouse monoclonal to FOXA1
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Knockout validated
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-FOXA1 antibody
See all FOXA1 primary antibodies -
Description
Mouse monoclonal to FOXA1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human FOXA1 aa 367-472.
Database link: P55317 -
Positive control
- Purchase matching WB positive control:Recombinant Human FOXA1 protein
- WB: HepG2 and HeLa cell lysates.
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General notes
This product was changed from ascites to tissue culture supernatant on 24/1/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4 -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-FOXA1 antibody (ab55178)All lanes : Anti-FOXA1 antibody (ab55178) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : FOXA1 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 49 kDaLanes 1- 2: Merged signal (red and green). Green - ab55178 observed at 49 kDa. Red - Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) observed at 37 kDa.
ab55178 was shown to react with FOXA1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261823 (knockout cell lysate ab256920) was used. Wild-type HeLa and FOXA1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab55178 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye®800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye®680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody (ab55178)FOXA1 antibody (ab55178) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human salivary gland.
This image was generated using the ascites version of the product.
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Immunocytochemistry/ Immunofluorescence - Anti-FOXA1 antibody (ab55178)ab55178 at 10 ug/ml staining FOXA1 in human HepG2 cells by Immunocytochemistry/ Immunofluorescence.
This image was generated using the ascites version of the product.
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Western blot - Anti-FOXA1 antibody (ab55178)FOXA1 antibody (ab55178) at 1ug/lane + HepG2 cell lysate at 25ug/lane.
This image was generated using the ascites version of the product.
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Flow Cytometry - Anti-FOXA1 antibody (ab55178)Overlay histogram showing HepG2 cells stained with ab55178 (red line). The cells were fixed with 4 % paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55178, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
