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Human ACSL4 (FACL4) knockout HeLa cell pellet (ab279199)

Overview

  • Product name

    Human ACSL4 (FACL4) knockout HeLa cell pellet
    See all FACL4 kits

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    HeLa

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon4.

  • Passage number

  • Knockout validation

    Sanger Sequencing

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Properties

Target

  • Function

    Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses arachidonate and eicosapentaenoate as substrates.

  • Involvement in disease

    Defects in ACSL4 are the cause of mental retardation X-linked type 63 (MRX63) [MIM:300387]. Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.
    Defects in ACSL4 are involved in Alport syndrome with mental retardation midface hypoplasia and elliptocytosis (ATS-MR) [MIM:300194]. A X-linked contiguous gene deletion syndrome characterized by glomerulonephritis, deafness, mental retardation, midface hypoplasia and elliptocytosis.

  • Sequence similarities

    Belongs to the ATP-dependent AMP-binding enzyme family.

  • Cellular localization

    Mitochondrion outer membrane. Peroxisome membrane. Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt

  • Alternative names

    • ACS 4
    • ACS4
    • ACSL 4
    • Acsl4
    • ACSL4_HUMAN
    • acyl CoA synthetase 4
    • Acyl CoA synthetase long chain family member 4
    • FACL 4
    • FACL4
    • Fatty acid Coenzyme A ligase
    • fatty acid Coenzyme A ligase long-chain 4
    • LACS 4
    • LACS4
    • Lignoceroyl CoA synthase
    • Long chain 4
    • long chain acyl CoA synthetase 4
    • long chain fatty acid CoA ligase 4
    • long chain fatty acid Coenzyme A ligase 4
    • Long-chain acyl-CoA synthetase 4
    • Long-chain-fatty-acid--CoA ligase 4
    • MRX63
    • MRX68
    see all

Properties

Images

  • Sanger Sequencing - Human ACSL4 (FACL4) knockout HeLa cell pellet (ab279199)
    Sanger Sequencing - Human ACSL4 (FACL4) knockout HeLa cell pellet (ab279199)
    Homozygous: Insertion of the selection cassette in exon4

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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