Anti-FOXA2 antibody (ab60721)
Key features and details
- Mouse monoclonal to FOXA2
- Suitable for: IP, WB, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-FOXA2 antibody
See all FOXA2 primary antibodies -
Description
Mouse monoclonal to FOXA2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB Human
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Immunogen
Recombinant fragment with tag: HLKPEHHYAF NHPFSINNLM SSEQQHHHSH HHHQPHKMDL KAYEQVMHYP GYGSPMPGSL AMGPVTNKTG LDASPLAADT SYYQGVYSRP IMNSS, corresponding to amino acids 363-458 of Human FOXA2
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Positive control
- HepG2 whole cell lysate (ab7900).
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General notes
This product was changed from ascites to tissue culture supernatant on 17/04/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Isotype
IgG2a -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-FOXA2 antibody (ab60721)
ab60721 at 10 ug/ml staining FOXA2 in human Hella cells by Immunocytochemistry/ Immunofluorescence.
This image was generated using the ascites version of the product.
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Western blot - Anti-FOXA2 antibody (ab60721)Anti-FOXA2 antibody (ab60721) at 1 µg/ml + HepG2 cell lysate at 25 µg
Predicted band size: 48 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa, 42 kDa. We are unsure as to the identity of these extra bands.This image was generated using the ascites version of the product.
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Flow Cytometry - Anti-FOXA2 antibody (ab60721)Overlay histogram showing HepG2 cells stained with ab60721 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab60721, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
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Immunoprecipitation - Anti-FOXA2 antibody (ab60721)Immunoprecipitation analysis of FOXA2 transfected lysate using ab60721 and protein A magnetic bead.
This image was generated using the ascites version of the product.
