Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FOXA2 antibody [EPR4465] - ChIP Grade (ab108396)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4465] to FOXA2 - ChIP Grade
  • Suitable for: WB, ChIP, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-FOXA2 antibody [EPR4465] - ChIP Grade
    See all FOXA2 primary antibodies

  • Description

    Rabbit monoclonal [EPR4465] to FOXA2 - ChIP Grade

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ChIP
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2 and Human colon cancer lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-FOXA2 antibody [EPR4465] - ChIP Grade (ab108396)
    Immunocytochemistry/ Immunofluorescence - Anti-FOXA2 antibody [EPR4465] - ChIP Grade (ab108396)

    Immunofluorescence staining of HepG2 cells with purified ab108396 at a working dilution of 1 in 300, counter-stained with DAPI. Tubulin was stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (ab150120) . The secondary antibody was ab150077 Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom middle and right hand panels - for the first negative control, purified ab108396 was used at a dilution of 1/200 followed by an Alexa Fluor® 555 goat anti-mouse antibody at a dilution of 1/500 and for the second negative control mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab15007) were used.

  • Western blot - Anti-FOXA2 antibody [EPR4465] - ChIP Grade (ab108396)
    Western blot - Anti-FOXA2 antibody [EPR4465] - ChIP Grade (ab108396)
    Anti-FOXA2 antibody [EPR4465] - ChIP Grade (ab108396) at 1/1000 dilution (purified) + HepG2 cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 48 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • ChIP - Anti-FOXA2 antibody [EPR4465] - ChIP Grade (ab108396)
    ChIP - Anti-FOXA2 antibody [EPR4465] - ChIP Grade (ab108396)
    Chromatin was prepared from HepG2 cells according to the Abcam Dual X-ChIP protocol*. Cells were fixed with EGS for 30 minutes, then formaldehyde for 10 minutes.
    The ChIP was performed with 25 µg of chromatin, 5 µg of ab108396 (red), and 20 µl of Protein A/G sepharose beads. 5 µg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
    Primers and probes are located in the first kb of the transcribed region.
    *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol
  • Western blot - Anti-FOXA2 antibody [EPR4465] - ChIP Grade (ab108396)
    Western blot - Anti-FOXA2 antibody [EPR4465] - ChIP Grade (ab108396)
    Anti-FOXA2 antibody [EPR4465] - ChIP Grade (ab108396) at 1/5000 dilution (purified) + SW480 cell lysate at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 48 kDa
    Observed band size: 52 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-FOXA2 antibody [EPR4465] - ChIP Grade (ab108396)
    Western blot - Anti-FOXA2 antibody [EPR4465] - ChIP Grade (ab108396)
    All lanes : Anti-FOXA2 antibody [EPR4465] - ChIP Grade (ab108396) at 1/1000 dilution

    Lane 1 : HepG2 lysate
    Lane 2 : Human colon cancer lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 48 kDa

  • Anti-FOXA2 antibody [EPR4465] - ChIP Grade (ab108396)
    Anti-FOXA2 antibody [EPR4465] - ChIP Grade (ab108396)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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