Anti-p53 antibody [TP53/1799R] (ab238070)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [TP53/1799R] to p53
- Suitable for: Protein Array, IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-p53 antibody [TP53/1799R]
See all p53 primary antibodies -
Description
Rabbit monoclonal [TP53/1799R] to p53 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanProtein Array HumanWB Human -
Immunogen
Recombinant full length protein corresponding to Human p53.
Database link: P04637 -
Positive control
- IHC-P: Human colon carcinoma tissue. WB: HeLa cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from Bioreactor concentrate. -
Clonality
Monoclonal -
Clone number
TP53/1799R -
Isotype
IgG -
Research areas
Images
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Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for p53 using ab238070 at 1 µg/mL in immunohistochemical analysis.
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Anti-p53 antibody [TP53/1799R] (ab238070) at 1 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Predicted band size: 43 kDa
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ab238070 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29. -