Native E. coli beta Galactosidase protein (Active) (ab7981)
Key features and details
- Expression system: Escherichia coli
- Active: Yes
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Product name
Native E. coli beta Galactosidase protein (Active)
See all beta Galactosidase proteins and peptides -
Biological activity
One Unit hydrolyzes one micromole of o-nitrophenyl-β-D-galactopyranoside per minute at 25°C, pH 7.5.
Activity: ≥50 units per mg dry weight.
Optimum pH range of 6 to 8.
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Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Native -
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Species
Escherichia coli -
Predicted molecular weight
540 kDa -
Additional sequence information
Beta Galactosidase is a homotetramer with a molecular weight of 465.4 kDa (116.3 kDa subunits each).
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Preparation and Storage
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Alternative names
- Beta D galactosidase
- Beta gal
- Beta galactosidase
see all -
Relevance
Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used. -
Cellular localization
Cytoplasmic
