Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-galectin 9/Gal-9 antibody [EPR23853-132] - BSA and Azide free (ab275886)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23853-132] to galectin 9/Gal-9 - BSA and Azide free
  • Suitable for: Flow Cyt, ICC, WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-galectin 9/Gal-9 antibody [EPR23853-132] - BSA and Azide free
    See all galectin 9/Gal-9 primary antibodies

  • Description

    Rabbit monoclonal [EPR23853-132] to galectin 9/Gal-9 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC, WBmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse thymus, liver and lung tissue lysates; EL4.IL-2, RAW 264.7 and NIH/3T3 whole cell lysates; Recombinant mouse galectin 9/Gal-9 protein. ICC: EL4.IL-2 cells. Flow cyt: EL4.IL-2 cells.

  • General notes

    ab275886 is the carrier-free version of ab275877. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab275886 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-galectin 9/Gal-9 antibody [EPR23853-132] - BSA and Azide free (ab275886)
    Western blot - Anti-galectin 9/Gal-9 antibody [EPR23853-132] - BSA and Azide free (ab275886)
    All lanes : Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877) at 1/1000 dilution

    Lane 1 : Mouse thymus tissue lysate
    Lane 2 : Mouse liver tissue lysate
    Lane 3 : Mouse lung tissue lysate
    Lane 4 : Mouse brain tissue lysate
    Lane 5 : EL4.IL-2 (mouse lymphoma t lymphocyte) whole cell lysate
    Lane 6 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 7 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 40 kDa
    Observed band size: 15,36,40 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab275877, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: Bands of 40KDa and 36KDa respectively represent two different isoforms.

    Band of 15KDa may represents the cleaved form.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 9038233, 11886844, 22805533, 15811318).

    Low expression: mouse brain (PMID: 9038233).

    Samples are non-boiled as boiling may cause protein aggregates.

    Exposure time: Lanes 1-2: 5.5 seconds; Lanes 3-6: 37 seconds; Lanes 7: 3 minutes.

     

  • Immunocytochemistry - Anti-galectin 9/Gal-9 antibody [EPR23853-132] - BSA and Azide free (ab275886)
    Immunocytochemistry - Anti-galectin 9/Gal-9 antibody [EPR23853-132] - BSA and Azide free (ab275886)

    This data was developed using ab275877, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized EL4.IL-2 cells labelling galectin 9/Gal-9 with ab275877 at 1/50 (12.28 µg/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous and cytoplasmic staining in EL4.IL-2 cell line. The staining pattern observed is consistent with what has been described in the literature (PMID: 28750861). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Flow Cytometry - Anti-galectin 9/Gal-9 antibody [EPR23853-132] - BSA and Azide free (ab275886)
    Flow Cytometry - Anti-galectin 9/Gal-9 antibody [EPR23853-132] - BSA and Azide free (ab275886)

    This data was developed using ab275877, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized EL4.IL-2 (mouse lymphoma T lymphocyte) cells labelling galectin 9/Gal-9 with ab275877 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730)  isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-galectin 9/Gal-9 antibody [EPR23853-132] - BSA and Azide free (ab275886)
    Western blot - Anti-galectin 9/Gal-9 antibody [EPR23853-132] - BSA and Azide free (ab275886)
    Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877) at 1/1000 dilution + Recombinant mouse galectin 9/Gal-9 protein, 20 ng

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 40 kDa



    This data was developed using ab275877, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds.

  • Anti-galectin 9/Gal-9 antibody [EPR23853-132] - BSA and Azide free (ab275886)
    Anti-galectin 9/Gal-9 antibody [EPR23853-132] - BSA and Azide free (ab275886)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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