Anti-Histone H1.4 (phospho T18) antibody (ab3595)
Key features and details
- Rabbit polyclonal to Histone H1.4 (phospho T18)
- Suitable for: WB, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Histone H1.4 (phospho T18) antibody
See all Histone H1.4 primary antibodies -
Description
Rabbit polyclonal to Histone H1.4 (phospho T18) -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human, Recombinant fragment
Predicted to work with: Mouse, Rat, Cow, Pig
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Immunogen
Synthetic peptide corresponding to Human Histone H1.4 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab10138)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab3595 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB 1/1000. Detects a band of approximately 32 kDa. ICC/IF Use at an assay dependent concentration. Target
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Function
Histones H1 are necessary for the condensation of nucleosome chains into higher order structures. -
Sequence similarities
Belongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain. -
Post-translational
modificationsAcetylated at Lys-26. Deacetylated at Lys-26 by SIRT1. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 3008 Human
- Entrez Gene: 50709 Mouse
- Omim: 142220 Human
- SwissProt: P10412 Human
- SwissProt: P43274 Mouse
- Unigene: 248133 Human
- Unigene: 170587 Mouse
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Alternative names
- H1 histone family member 4 antibody
- H1.4 antibody
- H14_HUMAN antibody
see all
Images
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Western blot - Anti-Histone H1.4 (phospho T18) antibody (ab3595)This image is courtesy of Alejandro Contreras, Baylor College of Medicine
Rabbit polyclonal to Histone H1 phospho T18 (1/1000) against recombinant Histone H1, incubated with control lysate (lanes 1, 3, 5, 7, 9, 11, 13) or insect cell lysate containing active cyclin E / CDK2 complexes (lanes 2, 4, 6, 8, 10, 12, 14).
Mutant Histone H1 proteins were made by site-directed mutagenesis of 5 phosphorylation cyclin/cdk phosphorylation concensus sites (T18, T146, T154, S172, S187). Lanes 3-12 contain mutant histone H1 with only one wild-type cyclin/cdk phosphorylation concensus site and lanes 13-14 containst mutant Histone H1 with all 5 sites mutated to Alanine.
Lanes 1-2 : wt Histone H1
Lanes 3-4 : T146A, T154A, S172A, S187A (wt site at T18)
Lanes 5-6 : T18A, T154A, S172A, S187A (wt site at T146)
Lanes 7-8 : T18A, T146A, S172A, S187A (wt site at T154)
Lanes 9-10 : T18A, T146A, T154A, S187A (wt site at S172)
Lanes 11-12: -
Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.4 (phospho T18) antibody (ab3595)This image is courtesy of Dr Michael Hendzels laboratoryRabbit polyclonal to Histone H1 (phospho T18) (1/25).
Human neuroblastoma cell line SK-N-SH cultured on coverslips were fixed in 4% paraformaldehye and then stained with ab3595 (green). The DNA stained with DAPI is shown in red. (100x magnification).
Protocols
Datasheets and documents
References (2)
ab3595 has been referenced in 2 publications.
- Chen Y et al. Quantitative Mass Spectrometry Reveals that Intact Histone H1 Phosphorylations are Variant Specific and Exhibit Single Molecule Hierarchical Dependence. Mol Cell Proteomics N/A:N/A (2015). PubMed: 26209608
- Terme JM et al. Dynamics and dispensability of variant-specific histone H1 Lys-26/Ser-27 and Thr-165 post-translational modifications. FEBS Lett 588:2353-62 (2014). PubMed: 24873882
Images
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Western blot - Anti-Histone H1.4 (phospho T18) antibody (ab3595) This image is courtesy of Alejandro Contreras, Baylor College of Medicine
Rabbit polyclonal to Histone H1 phospho T18 (1/1000) against recombinant Histone H1, incubated with control lysate (lanes 1, 3, 5, 7, 9, 11, 13) or insect cell lysate containing active cyclin E / CDK2 complexes (lanes 2, 4, 6, 8, 10, 12, 14).
Mutant Histone H1 proteins were made by site-directed mutagenesis of 5 phosphorylation cyclin/cdk phosphorylation concensus sites (T18, T146, T154, S172, S187). Lanes 3-12 contain mutant histone H1 with only one wild-type cyclin/cdk phosphorylation concensus site and lanes 13-14 containst mutant Histone H1 with all 5 sites mutated to Alanine.
Lanes 1-2 : wt Histone H1
Lanes 3-4 : T146A, T154A, S172A, S187A (wt site at T18)
Lanes 5-6 : T18A, T154A, S172A, S187A (wt site at T146)
Lanes 7-8 : T18A, T146A, S172A, S187A (wt site at T154)
Lanes 9-10 : T18A, T146A, T154A, S187A (wt site at S172)
Lanes 11-12: -
Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.4 (phospho T18) antibody (ab3595) This image is courtesy of Dr Michael Hendzels laboratory
Rabbit polyclonal to Histone H1 (phospho T18) (1/25).
Human neuroblastoma cell line SK-N-SH cultured on coverslips were fixed in 4% paraformaldehye and then stained with ab3595 (green). The DNA stained with DAPI is shown in red. (100x magnification).
