Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

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Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18472] to KAT9 / Elp3
Suitable for: IP, ICC/IF, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-KAT9 / Elp3 antibody [EPR18472]
See all KAT9 / Elp3 primary antibodies
Description

Rabbit monoclonal [EPR18472] to KAT9 / Elp3
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, Jurkat, 293, HepG2, MCF7, C6, RAW264.7, PC-12 and NIH/3T3 whole cell lysates; Human fetal brain, fetal heart and fetal kidney lysates; Mouse and rat brain, heart, kidney and spleen lysates. ICC/IF: HeLa cells. IP: MCF7 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18472
Isotype

IgG
Research areas

Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907)

All lanes : Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907) at 1/5000 dilution

Lanes 1 & 7 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lane 4 : 293 (Human epithelial cells from embryonic kidney) whole cell lysate
Lane 5 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

Predicted band size: 62 kDa
Observed band size: 62 kDa

Exposure time: 5 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907)

All lanes : Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907) at 1/2000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 62 kDa
Observed band size: 62 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907)

All lanes : Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907) at 1/2000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Mouse spleen lysate
Lane 5 : Rat brain lysate
Lane 6 : Rat heart lysate
Lane 7 : Rat kidney lysate
Lane 8 : Rat spleen lysate
Lane 9 : C6 (Rat glial tumor cells) whole cell lysate
Lane 10 : RAW264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 11 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 12 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

Predicted band size: 62 kDa
Observed band size: 62 kDa

Exposure time: 5 seconds

5% NFDM/TBST: Blocking and diluting buffer.
Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907)

All lanes : Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907) at 1/10000 dilution

Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) cytosolic fraction lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) nuclear fraction lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

Predicted band size: 62 kDa
Observed band size: 62 kDa

Exposure time: 15 seconds
Immunocytochemistry/ Immunofluorescence - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling KAT9 / Elp3 with ab190907 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic and nuclear staining on HeLa cells line.The staining pattern is in agreement with the data shown in the following publications: Cell 2009. 136, 551-64 (PMID: 19185337) and J Biol Chem. 2002. 277, 3047-3052 (PMID: 11714725).
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab190907 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
Immunoprecipitation - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907)

KAT9 / Elp3 was immunoprecipitated from 1mg of MCF7 (Human breast adenocarcinoma cell line) whole cell lysate with ab190907 at 1/70 dilution.
Lane 1: MCF7 whole cell lysate 10ug (Input).
Lane 2: ab190907 IP in MCF7 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab190907 in MCF7 whole cell lysate.
Western blot was performed from the immunoprecipitate using ab190907 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907)

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