Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907)
![Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907)](https://www.abcam.com/ps/products/190/ab190907/Images/ab190907-247053-anti-kat9-elp3-antibody-epr18472-western-blot.jpg "Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18472] to KAT9 / Elp3
- Suitable for: IP, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-KAT9 / Elp3 antibody [EPR18472]
See all KAT9 / Elp3 primary antibodies -
Description
Rabbit monoclonal [EPR18472] to KAT9 / Elp3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIP HumanWB MouseRatHuman
-
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HeLa, Jurkat, 293, HepG2, MCF7, C6, RAW264.7, PC-12 and NIH/3T3 whole cell lysates; Human fetal brain, fetal heart and fetal kidney lysates; Mouse and rat brain, heart, kidney and spleen lysates. ICC/IF: HeLa cells. IP: MCF7 whole cell lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18472 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907)All lanes : Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907) at 1/5000 dilution
Lanes 1 & 7 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lane 4 : 293 (Human epithelial cells from embryonic kidney) whole cell lysate
Lane 5 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Predicted band size: 62 kDa
Observed band size: 62 kDa
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907)](https://www.abcam.com/ps/products/190/ab190907/Images/ab190907-247050-anti-kat9-elp3-antibody-epr18472-western-blot.jpg)
Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907)All lanes : Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907) at 1/2000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 62 kDa
Observed band size: 62 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907)](https://www.abcam.com/ps/products/190/ab190907/Images/ab190907-247045-anti-kat9-elp3-antibody-epr18472-western-blot.jpg)
Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907)All lanes : Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907) at 1/2000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Mouse spleen lysate
Lane 5 : Rat brain lysate
Lane 6 : Rat heart lysate
Lane 7 : Rat kidney lysate
Lane 8 : Rat spleen lysate
Lane 9 : C6 (Rat glial tumor cells) whole cell lysate
Lane 10 : RAW264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 11 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 12 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Predicted band size: 62 kDa
Observed band size: 62 kDa
Exposure time: 5 seconds5% NFDM/TBST: Blocking and diluting buffer.
-
![Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907)](https://www.abcam.com/ps/products/190/ab190907/Images/ab190907-247041-anti-kat9-elp3-antibody-epr18472-western-blot.jpg)
Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907)All lanes : Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907) at 1/10000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) cytosolic fraction lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) nuclear fraction lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Predicted band size: 62 kDa
Observed band size: 62 kDa
Exposure time: 15 seconds
-
![Immunocytochemistry/ Immunofluorescence - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907)](https://www.abcam.com/ps/products/190/ab190907/Images/ab190907-247039-anti-kat9-elp3-antibody-epr18472-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling KAT9 / Elp3 with ab190907 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic and nuclear staining on HeLa cells line.The staining pattern is in agreement with the data shown in the following publications: Cell 2009. 136, 551-64 (PMID: 19185337) and J Biol Chem. 2002. 277, 3047-3052 (PMID: 11714725).
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab190907 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution. -
![Immunoprecipitation - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907)](https://www.abcam.com/ps/products/190/ab190907/Images/ab190907-247038-anti-kat9-elp3-antibody-epr18472-immunoprecipitation.jpg)
Immunoprecipitation - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907)KAT9 / Elp3 was immunoprecipitated from 1mg of MCF7 (Human breast adenocarcinoma cell line) whole cell lysate with ab190907 at 1/70 dilution.
Lane 1: MCF7 whole cell lysate 10ug (Input).
Lane 2: ab190907 IP in MCF7 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab190907 in MCF7 whole cell lysate.
Western blot was performed from the immunoprecipitate using ab190907 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
-
![Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907)](https://www.abcam.com/ps/products/190/ab190907/Images/ab190907-7-benefits-of-recombinant-antibodies.png)
Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907)
