Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [RM216] (ab222765)
![Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [RM216] (ab222765)](https://www.abcam.com/ps/products/222/ab222765/Images/ab222765-282532-anti-histone-h2a-phospho-s1-histone-h4-phospho-s1-antibody-rm216-western-blot.jpg "Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [RM216] (ab222765)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM216] to Histone H2A (phospho S1) + Histone H4 (phospho S1)
- Suitable for: ICC/IF, WB
- Reacts with: Human
Overview
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Product name
Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [RM216] -
Description
Rabbit monoclonal [RM216] to Histone H2A (phospho S1) + Histone H4 (phospho S1) -
Host species
Rabbit -
Specificity
ab222765 reacts to Histone H4 or Histone H2A phosphorylated at Serine 1. No cross reactivity with other phosphorylated Histones. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human Histone H2A (phospho S1).
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Positive control
- WB: Acid extract of HeLa cells treated with Nocodazole. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
RM216 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [RM216] (ab222765)All lanes : Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [RM216] (ab222765) at 0.5 µg/ml
Lane 1 : Acid extract of HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with Nocodazole
Lane 2 : Acid extract of HeLa (human epithelial cell line from cervix adenocarcinoma) cells non-treated with Nocodazole
Predicted band size: 14, 11 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [RM216] (ab222765)](https://www.abcam.com/ps/products/222/ab222765/Images/ab222765-282533-anti-histone-h2a-phospho-s1-histone-h4-phospho-s1-antibody-rm216-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [RM216] (ab222765)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Histone H2A (phospho S1) + Histone H4 (phospho S1) (red) using ab222765 at 2 µg/ml in ICC/IF. Actin filaments have been labeled with fluorescein phalloidin (green).
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![Biological activity - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [RM216] (ab222765)](https://www.abcam.com/ps/products/222/ab222765/Images/ab222765-282531-anti-histone-h2a-phospho-s1-histone-h4-phospho-s1-antibody-rm216.jpg)
Biological activity - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [RM216] (ab222765)ab222765 specifically reacts to both Histone H2A and H4 phosphorylated at Serine 1 (H2AS1p and H4S1p).
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![Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [RM216] (ab222765)](https://www.abcam.com/ps/products/222/ab222765/Images/ab222765-4-benefits-of-recombinant-antibodies.png)
Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [RM216] (ab222765)
