Anti-Histone H3 (phospho S10 + S28) antibody (ab46986)
Key features and details
- Rabbit polyclonal to Histone H3 (phospho S10 + S28)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Histone H3 (phospho S10 + S28) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (phospho S10 + S28) -
Host species
Rabbit -
Specificity
Specificity was determined by dot blots with a non-modified histone H3 peptide and peptides phosphorylated at serines 10 or 28. This antibody recognizes histone H3 phosphorylated at serine 10 and/or serine 28. Serines 10 and 28 are highly phosphorylated during mitosis. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide corresponding to Human Histone H3 (phospho S10 + S28).
Database link: P68431 -
Positive control
- HeLa cells.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.035% Sodium azide
Constituent: 30% Glycerol -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Histone H3 (phospho S10 + S28) antibody (ab46986)All lanes : Anti-Histone H3 (phospho S10 + S28) antibody (ab46986) at 1/5000 dilution
Lane 1 : Acid extract of HeLa cells, no treatment
Lane 2 : Acid extract of HeLa cells, treatment with colcemid.
Predicted band size: 16 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
