Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Growth hormone receptor antibody [EPR5291(2)] (ab134078)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5291(2)] to Growth hormone receptor
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Growth hormone receptor antibody [EPR5291(2)]
    See all Growth hormone receptor primary antibodies

  • Description

    Rabbit monoclonal [EPR5291(2)] to Growth hormone receptor

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Growth hormone receptor aa 550-650 (intracellular). The exact sequence is proprietary.

  • Positive control

    • WB: MCF-7, BxPC-3, and human fetal muscle, mouse brain and rat brain lysate. ICC/IF: HepG2 cells. Flow Cyt: MCF-7 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Growth hormone receptor antibody [EPR5291(2)] (ab134078)
    Western blot - Anti-Growth hormone receptor antibody [EPR5291(2)] (ab134078)
    Anti-Growth hormone receptor antibody [EPR5291(2)] (ab134078) at 1/2000 dilution (purified) + BxPC-3 cell lysate at 10 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 72 kDa
    Observed band size: 140 kDa
    why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-Growth hormone receptor antibody [EPR5291(2)] (ab134078)
    Western blot - Anti-Growth hormone receptor antibody [EPR5291(2)] (ab134078)
    Anti-Growth hormone receptor antibody [EPR5291(2)] (ab134078) at 1/10000 dilution (purified) + MCF-7 cell lysate at 10 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 72 kDa
    Observed band size: 140 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-Growth hormone receptor antibody [EPR5291(2)] (ab134078)
    Western blot - Anti-Growth hormone receptor antibody [EPR5291(2)] (ab134078)
    All lanes : Anti-Growth hormone receptor antibody [EPR5291(2)] (ab134078) at 1/10000 dilution (purified)

    Lane 1 : Mouse brain
    Lane 2 : Rat brain

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 72 kDa
    Observed band size: 140 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-Growth hormone receptor antibody [EPR5291(2)] (ab134078)
    Immunocytochemistry/ Immunofluorescence - Anti-Growth hormone receptor antibody [EPR5291(2)] (ab134078)

    Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling Growth hormone receptor with purified ab134078 at 1/200. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

    Control: primary antibody (1/500) and secondary antibody ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/400).

  • Immunocytochemistry/ Immunofluorescence - Anti-Growth hormone receptor antibody [EPR5291(2)] (ab134078)
    Immunocytochemistry/ Immunofluorescence - Anti-Growth hormone receptor antibody [EPR5291(2)] (ab134078) This image is courtesy of an anonymous Abreview

    ab134078 (unpurified) staining Growth hormone receptor in a human glioblastoma cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde,permeabilized with TBS Tween 0.5% and blocked for 60 minutes at 25°C. Samples were incubated with primary antibody (1/100) for 20 hour at 4°C. An Alexa Fluor® 594-conjugated Goat anti-rabbit IgG polyclonal(1/200) was used as the secondary antibody.

    See Abreview

  • Flow Cytometry - Anti-Growth hormone receptor antibody [EPR5291(2)] (ab134078)
    Flow Cytometry - Anti-Growth hormone receptor antibody [EPR5291(2)] (ab134078)

    Flow cytometry analysis of MCF-7 cells labelling Growth hormone receptor with purified ab134078 at 1/420 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • OI-RD Scanning - Anti-Growth hormone receptor antibody [EPR5291(2)] (ab134078)
    OI-RD Scanning - Anti-Growth hormone receptor antibody [EPR5291(2)] (ab134078)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Growth hormone receptor antibody [EPR5291(2)] (ab134078)
    Anti-Growth hormone receptor antibody [EPR5291(2)] (ab134078)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Growth hormone receptor antibody [EPR5291(2)] (ab134078)

© 2021 Astana Biomed Group LLP. All rights reserved.