Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Growth hormone receptor antibody (ab65304)

Key features and details

  • Rabbit polyclonal to Growth hormone receptor
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Growth hormone receptor antibody
    See all Growth hormone receptor primary antibodies

  • Description

    Rabbit polyclonal to Growth hormone receptor

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Growth hormone receptor.

    Read Abcam's proprietary immunogen policy (Peptide available as ab73395.)

  • Positive control

    • This antibody gave a positive result in the following whole cell lysates: A549, HeLa, DU 145

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab65304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
All applications
Non human primates
Application Abreviews Notes
ICC/IF (1)
Use a concentration of 5 µg/ml.
WB (1)
Use a concentration of 1 µg/ml. Detects a band of approximately 76 kDa (predicted molecular weight: 71 kDa).
Notes
ICC/IF
Use a concentration of 5 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 76 kDa (predicted molecular weight: 71 kDa).

Target

  • Function

    Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway.
    The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling.
    Isoform 2 up-regulates the production of GHBP and acts as a negative inhibitor of GH signaling.

  • Tissue specificity

    Expressed in various tissues with high expression in liver and skeletal muscle. Isoform 4 is predominantly expressed in kidney, bladder, adrenal gland and brain stem. Isoform 1 expression in placenta is predominant in chorion and decidua. Isoform 4 is highly expressed in placental villi. Isoform 2 is expressed in lung, stomach and muscle. Low levels in liver.

  • Involvement in disease

    Defects in GHR are a cause of Laron syndrome (LARS) [MIM:262500]. A severe form of growth hormone insensitivity characterized by growth impairment, short stature, dysfunctional growth hormone receptor, and failure to generate insulin-like growth factor I in response to growth hormone.
    Defects in GHR may be a cause of idiopathic short stature autosomal (ISSA) [MIM:604271]. Short stature is defined by a subnormal rate of growth.

  • Sequence similarities

    Belongs to the type I cytokine receptor family. Type 1 subfamily.
    Contains 1 fibronectin type-III domain.

  • Domain

    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
    The extracellular domain is the ligand-binding domain representing the growth hormone-binding protein (GHBP).
    The ubiquitination-dependent endocytosis motif (UbE) is required for recruitment of the ubiquitin conjugation system on to the receptor and for its internalization.

  • Post-translational
    modifications

    The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. Shedding is inhibited by growth hormone (GH) binding to the receptor probably due to a conformational change in GHR rendering the receptor inaccessible to ADAM17.
    On GH binding, phosphorylated on tyrosine residues in the cytoplasmic domain by JAK2.
    On ligand binding, ubiquitinated on lysine residues in the cytoplasmic domain. This ubiquitination is not sufficient for GHR internalization.

  • Cellular localization

    Secreted; Cell membrane. On growth hormone binding, GHR is ubiquitinated, internalized, down-regulated and transported into a degradative or non-degradative pathway and Cell membrane. Remains fixed to the cell membrane and is not internalized.
  • Information by UniProt

  • Database links

    • Alternative names

      • GH receptor antibody
      • GH-binding protein antibody
      • GHBP antibody
      • GHBP, included antibody
      • GHR antibody
      • GHR_HUMAN antibody
      • Growth hormone binding protein antibody
      • Growth hormone receptor antibody
      • Growth hormone receptor precursor antibody
      • Growth hormone-binding protein antibody
      • Growth hormone-binding protein, included antibody
      • Increased responsiveness to growth hormone, included antibody
      • Serum binding protein antibody
      • Serum-binding protein antibody
      • Somatotropin receptor antibody
      see all

    Images

    • Western blot - Anti-Growth hormone receptor antibody (ab65304)
      Western blot - Anti-Growth hormone receptor antibody (ab65304)
      All lanes : Anti-Growth hormone receptor antibody (ab65304) at 1 µg/ml

      Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 3 : DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 71 kDa
      Observed band size: 76 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 56 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 12 minutes


      Growth hormone receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
    • Immunocytochemistry/ Immunofluorescence - Anti-Growth hormone receptor antibody (ab65304)
      Immunocytochemistry/ Immunofluorescence - Anti-Growth hormone receptor antibody (ab65304)
      ICC/IF image of ab65304 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65304, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, Hek293 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.

    References (1)

    Publishing research using ab65304? Please let us know so that we can cite the reference in this datasheet.

    ab65304 has been referenced in 1 publication.

    • Beltran-Sastre V  et al. Tuneable endogenous mammalian target complementation via multiplexed plasmid-based recombineering. Sci Rep 5:17432 (2015). WB ; Human . PubMed: 26612112

    Images

    • Western blot - Anti-Growth hormone receptor antibody (ab65304)
      Western blot - Anti-Growth hormone receptor antibody (ab65304)
      All lanes : Anti-Growth hormone receptor antibody (ab65304) at 1 µg/ml

      Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 3 : DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 71 kDa
      Observed band size: 76 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 56 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 12 minutes


      Growth hormone receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
    • Immunocytochemistry/ Immunofluorescence - Anti-Growth hormone receptor antibody (ab65304)
      Immunocytochemistry/ Immunofluorescence - Anti-Growth hormone receptor antibody (ab65304)
      ICC/IF image of ab65304 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65304, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, Hek293 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.

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