Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12084(B)] to HSD17B8
- Suitable for: ICC/IF, WB, IP
- Reacts with: Human
Overview
-
Product name
Anti-HSD17B8 antibody [EPR12084(B)]
See all HSD17B8 primary antibodies -
Description
Rabbit monoclonal [EPR12084(B)] to HSD17B8 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human -
Immunogen
Synthetic peptide corresponding to amino acid residues in Human HSD17B8 (Q92506).
-
Positive control
- Human fetal liver, HepG2, BxPC-3 and JAR cell lysates. JAR cells.
-
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12084(B) -
Isotype
IgG -
Research areas
Images
-
Immunofluorescent analysis of JAR cells labelling HSD17B8 with ab169752 at 1/100 dilution.
-
All lanes : Anti-HSD17B8 antibody [EPR12084(B)] (ab169752) at 1/10000 dilution
Lane 1 : Fetal liver lysate
Lane 2 : HepG2 lysate
Lane 3 : Bx-PC-3 lysate
Lane 4 : JAR lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 26 kDa
-
Western blot analysis on immunoprecipitation pellet from (1) fetal liver lysate labeling HSD17B8 with ab169752 at 1/10 dilution or (2) 1X PBS (negative control. HRP-conjugated anti-rabbit IgG preferentially detected the non-reduced form of Rabbit IgG.
-