Anti-ACAA2 antibody [EPR6733] - BSA and Azide free (ab248216)
![Anti-ACAA2 antibody [EPR6733] - BSA and Azide free (ab248216)](https://www.abcam.com/ps/products/248/ab248216/Images/ab248216-407770-anti-acaa2-antibody-epr6733-bsa-and-azide-free-western-blot.jpg "Anti-ACAA2 antibody [EPR6733] - BSA and Azide free (ab248216)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6733] to ACAA2 - BSA and Azide free
- Suitable for: IHC-P, ICC, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ACAA2 antibody [EPR6733] - BSA and Azide free
See all ACAA2 primary antibodies -
Description
Rabbit monoclonal [EPR6733] to ACAA2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC, WBmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab248216 is the carrier-free version of ab128911. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab248216 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 2.30 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR6733 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ACAA2 antibody [EPR6733] - BSA and Azide free (ab248216)All lanes : Anti-ACAA2 antibody [EPR6733] (ab128911) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 41 kDaThis data was developed using ab128911, the same antibody clone in a different buffer formulation.
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![Immunocytochemistry - Anti-ACAA2 antibody [EPR6733] - BSA and Azide free (ab248216)](https://www.abcam.com/ps/products/248/ab248216/Images/ab248216-3-anti-acaa2-antibody-epr6733-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-ACAA2 antibody [EPR6733] - BSA and Azide free (ab248216)This data was developed using ab128911, the same antibody clone in a different buffer formulation.ab128911 stained HepG2 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab128911 at 1/100 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA2 antibody [EPR6733] - BSA and Azide free (ab248216)](https://www.abcam.com/ps/products/248/ab248216/Images/ab248216-2-anti-acaa2-antibody-epr6733-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA2 antibody [EPR6733] - BSA and Azide free (ab248216)This data was developed using ab128911, the same antibody clone in a different buffer formulation.ab128911 at 1/100 dilution staining ACAA2 in paraffin embedded Human colonic carcinoma tissue by immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![OI-RD Scanning - Anti-ACAA2 antibody [EPR6733] - BSA and Azide free (ab248216)](https://www.abcam.com/ps/products/248/ab248216/Images/ab248216-1-anti-acaa2-antibody-epr6733-bsa-and-azide-free-equilibrium-disassociation-constant-kd.jpg)
OI-RD Scanning - Anti-ACAA2 antibody [EPR6733] - BSA and Azide free (ab248216)This data was developed using ab128911, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
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![Anti-ACAA2 antibody [EPR6733] - BSA and Azide free (ab248216)](https://www.abcam.com/ps/products/248/ab248216/Images/ab248216-5-benefits-of-recombinant-antibodies.png)
Anti-ACAA2 antibody [EPR6733] - BSA and Azide free (ab248216)