Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2017] to Retinoic Acid Receptor beta
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-Retinoic Acid Receptor beta antibody [EPR2017]
    See all Retinoic Acid Receptor beta primary antibodies

  • Description

    Rabbit monoclonal [EPR2017] to Retinoic Acid Receptor beta

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Retinoic Acid Receptor beta aa 400 to the C-terminus.

  • Positive control

    • WB: Daudi, MCF7, MDA-MB-435, and U-87 MG lysates. IHC-P: human bladder carcinoma and kidney tissues. ICC/IF: HCT 116 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)
    Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)
    Immunocytochemistry/ Immunofluorescence analysis of HCT 116 (Human colorectal carcinoma epithelial cell) cells labeling Retinoic Acid Receptor beta with Purified ab124701 at 1:100 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue sections labeling Retinoic Acid Receptor beta with Purified ab124701 at 1:100 dilution (10.3 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)
    Western blot - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)
    All lanes : Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701) at 1/2000 dilution (Purified)

    Lane 1 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysates
    Lane 2 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 50 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)
    Western blot - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)
    All lanes : Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701) at 1/1000 dilution

    Lane 1 : MCF7 cell lysates
    Lane 2 : MDA-MB-435 cell lysates
    Lane 3 : Daudi cell lysates
    Lane 4 : U-87 MG cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lane 1 : Goat anti-Rabbit HRP at 1/20000 dilution
    Lanes 2-4 : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 50 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)

    ab124701 at 1/100 dilution staining Retinoic Acid Receptor beta in paraffin-embedded Human kidney tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)
    OI-RD Scanning - Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)
    Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)

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