Anti-Retinoic Acid Receptor beta antibody (ab53161)
Key features and details
- Rabbit polyclonal to Retinoic Acid Receptor beta
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Retinoic Acid Receptor beta antibody
See all Retinoic Acid Receptor beta primary antibodies -
Description
Rabbit polyclonal to Retinoic Acid Receptor beta -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide, corresponding to amino acids 351-390 of Human Retinoic Acid Receptor beta
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Retinoic Acid Receptor beta antibody (ab53161)All lanes : Anti-Retinoic Acid Receptor beta antibody (ab53161) at 1/500 dilution
Lane 1 : HepG2 cell extract, untreated.
Lane 2 : HepG2 cell extract treated with the immunising peptide.
Predicted band size: 50 kDa
Observed band size: 50 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoic Acid Receptor beta antibody (ab53161)This image shows human breast carcinoma tissue stained with ab53161 at 1/50 dilution. The left hand image shows untreated tissue; the right hand image shows tissue treated with the immunising peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor beta antibody (ab53161)ICC/IF image of ab53161 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53161, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
