Recombinant human EZH2 + EED + SUZ12 + AEBP2 + RBBP4 (mutated A677G) protein (ab198114)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 82% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus, DDDDK tag N-Terminus, His tag N-Terminus, His tag N-Terminus, His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human EZH2 + EED + SUZ12 + AEBP2 + RBBP4 (mutated A677G) protein
See all EZH2 + EED + SUZ12 + AEBP2 + RBBP4 proteins and peptides -
Biological activity
ab198114 is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
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Purity
>= 82 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Amino Acid Sequence 1
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Species
Human -
Predicted molecular weight
86 kDa including tags -
Amino acids
2 to 751 -
Modifications
mutated A677G -
Tags
His tag N-Terminus -
Additional sequence information
GenBank Accession No. NM_004456
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Amino Acid Sequence 2
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Species
Human -
Sequence
SEREVSTAPAGTDMPAAKKQKLSSDENSNPDLSGDENDDAVSIESGTNTE RPDTPTNTPNAPGRKSWGKGKWKSKKCKYSFKCVNSLKEDHNQPLFGVQF NWHSKEGDPLVFATVGSNRVTLYECHSQGEIRLLQSYVDADADENFYTCA WTYDSNTSHPLLAVAGSRGIIRIINPITMQCIKHYVGHGNAINELKFHPR DPNLLLSVSKDHALRLWNIQTDTLVAIFGGVEGHRDEVLSADYDLLGEKI MSCGMDHSLKLWRINSKRMMNAIKESYDYNPNKTNRPFISQKIHFPDFST RDIHRNYVDCVRWLGDLILSKSCENAIVCWKPGKMEDDIDKIKPSESNVT ILGRFDYSQCDIWYMRFSMDFWQKMLALGNQVGKLYVWDLEVEDPHKAKC TTLTHHKCGAAIRQTSFSRDSSILIAVCDDASIWRWDRLR -
Predicted molecular weight
51 kDa including tags -
Amino acids
2 to 441 -
Tags
DDDDK tag N-Terminus -
Additional sequence information
EED, GenBank Accession No. NM_003797
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Amino Acid Sequence 3
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Species
Human -
Sequence
APQKHGGGGGGGSGPSAGSGGGGFGGSAAVAAATASGGKSGGGSCGGGGS YSASSSSSAAAAAGAAVLPVKKPKMEHVQADHELFLQAFEKPTQIYRFLR TRNLIAPIFLHRTLTYMSHRNSRTNIKRKTFKVDDMLSKVEKMKGEQESH SLSAHLQLTFTGFFHKNDKPSPNSENEQNSVTLEVLLVKVCHKKRKDVSC PIRQVPTGKKQVPLNPDLNQTKPGNFPSLAVSSNEFEPSNSHMVKSYSLL FRVTRPGRREFNGMINGETNENIDVNEELPARRKRNREDGEKTFVAQMTV FDKNRRLQLLDGEYEVAMQEMEECPISKKRATWETILDGKRLPPFETFSQ GPTLQFTLRWTGETNDKSTAPIAKPLATRNSESLHQENKPGSVKPTQTIA VKESLTTDLQTRKEKDTPNENRQKLRIFYQFLYNNNTRQQTEARDDLHCP WCTLNCRKLYSLLKHLKLCHSRFIFNYVYHPKGARIDVSINECYDGSYAG NPQDIHRQPGFAFSRNGPVKRTPITHILVCRPKRTKASMSEFLESEDGEV EQQRTYSSGHNRLYFHSDTCLPLRPQEMEVDSEDEKDPEWLREKTITQIE EFSDVNEGEKEVMKLWNLHVMKHGFIADNQMNHACMLFVENYGQKIIKKN LCRNFMLHLVSMHDFNLISIMSIDKAVTKLREMQQKLEKGESASPANEEI TEEQNGTANGFSEINSKEKALETDSVSGVSKQSKKQKL -
Predicted molecular weight
87 kDa including tags -
Amino acids
2 to 739 -
Tags
His tag N-Terminus -
Additional sequence information
SUZ12, GenBank Accession No. NM_015355
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Amino Acid Sequence 4
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Species
Human -
Sequence
AAAITDMADLEELSRLSPLPPGSPGSAARGRAEPPEEEEEEEEEEEEAEA EAVAALLLNGGSGGGGGGGGGGVGGGEAETMSEPSPESASQAGEDEDEEE DDEEEEDESSSSGGGEEESSAESLVGSSGGSSSDETRSLSPGAASSSSGD GDGKEGLEEPKGPRGSQGGGGGGSSSSSVVSSGGDEGYGTGGGGSSATSG GRRGSLEMSSDGEPLSRMDSEDSISSTIMDVDSTISSGRSTPAMMNGQGS TTSSSKNIAYNCCWDQCQACFNSSPDLADHIRSIHVDGQRGGVFVCLWKG CKVYNTPSTSQSWLQRHMLTHSGDKPFKCVVGGCNASFASQGGLARHVPT HFSQQNSSKVSSQPKAKEESPSKAGMNKRRKLKNKRRRSLPRPHDFFDAQ TLDAIRHRAICFNLSAHIESLGKGHSVVFHSTVIAKRKEDSGKIKLLLHW MPEDILPDVWVNESERHQLKTKVVHLSKLPKDTALLLDPNIYRTMPQKRL KRTLIRKVFNLYLSKQ -
Predicted molecular weight
53 kDa including tags -
Amino acids
2 to 517 -
Tags
His tag N-Terminus -
Additional sequence information
AEBP2, GenBank Accession No. NM_153207
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Amino Acid Sequence 5
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Species
Human -
Sequence
ADKEAAFDDAVEERVINEEYKIWKKNTPFLYDLVMTHALEWPSLTAQWLP DVTRPEGKDFSIHRLVLGTHTSDEQNHLVIASVQLPNDDAQFDASHYDSE KGEFGGFGSVSGKIEIEIKINHEGEVNRARYMPQNPCIIATKTPSSDVLV FDYTKHPSKPDPSGECNPDLRLRGHQKEGYGLSWNPNLSGHLLSASDDHT ICLWDISAVPKEGKVVDAKTIFTGHTAVVEDVSWHLLHESLFGSVADDQK LMIWDTRSNNTSKPSHSVDAHTAEVNCLSFNPYSEFILATGSADKTVALW DLRNLKLKLHSFESHKDEIFQVQWSPHNETILASSGTDRRLNVWDLSKIG EEQSPEDAEDGPPELLFIHGGHTAKISDFSWNPNEPWVICSVSEDNIMQV WQMAENIYNDEDPEGSVDPEGQGS -
Predicted molecular weight
48 kDa including tags -
Amino acids
2 to 425 -
Tags
His tag N-Terminus -
Additional sequence information
RBBP4, GenBank Accession No. NM_005610
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Preparation and Storage
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Amino Acid Sequence 5
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Species
Human -
Sequence
ADKEAAFDDAVEERVINEEYKIWKKNTPFLYDLVMTHALEWPSLTAQWLP DVTRPEGKDFSIHRLVLGTHTSDEQNHLVIASVQLPNDDAQFDASHYDSE KGEFGGFGSVSGKIEIEIKINHEGEVNRARYMPQNPCIIATKTPSSDVLV FDYTKHPSKPDPSGECNPDLRLRGHQKEGYGLSWNPNLSGHLLSASDDHT ICLWDISAVPKEGKVVDAKTIFTGHTAVVEDVSWHLLHESLFGSVADDQK LMIWDTRSNNTSKPSHSVDAHTAEVNCLSFNPYSEFILATGSADKTVALW DLRNLKLKLHSFESHKDEIFQVQWSPHNETILASSGTDRRLNVWDLSKIG EEQSPEDAEDGPPELLFIHGGHTAKISDFSWNPNEPWVICSVSEDNIMQV WQMAENIYNDEDPEGSVDPEGQGS -
Predicted molecular weight
48 kDa including tags -
Amino acids
2 to 425 -
Tags
His tag N-Terminus -
Additional sequence information
RBBP4, GenBank Accession No. NM_005610
Images
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SDS-PAGE - Recombinant human EZH2 + EED + SUZ12 + AEBP2 + RBBP4 (mutated A677G) protein (ab198114)
4-20% SDS-PAGE analysis of ab198114.
Lane 1: 3.16 µg ab198114 enzyme complex
Lane 2: Protein markerStained with Coomassie blue.
