Anti-ZNF549 antibody (ab224661)
Key features and details
- Rabbit polyclonal to ZNF549
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ZNF549 antibody
See all ZNF549 primary antibodies -
Description
Rabbit polyclonal to ZNF549 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human ZNF549 aa 1-280.
Database link: Q6P9A3 -
Positive control
- WB: A549 and HeLa whole cell lysates; Human placenta tissue lysate. IHC-P: Human gastric cancer and breast cancer tissues.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF549 antibody (ab224661)
Paraffin-embedded human gastric cancer tissue stained for ZNF549 using ab224661 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF549 antibody (ab224661)Paraffin-embedded human breast cancer tissue stained for ZNF549 using ab224661 at 1/100 dilution in immunohistochemical analysis.
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Western blot - Anti-ZNF549 antibody (ab224661)All lanes : Anti-ZNF549 antibody (ab224661) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : Human placenta tissue lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 73,75 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
