Anti-PML+RARA Fusion antibody (ab43152)
Key features and details
- Rabbit polyclonal to PML+RARA Fusion
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PML+RARA Fusion antibody -
Description
Rabbit polyclonal to PML+RARA Fusion -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human PML+RARA Fusion aa 500-600 conjugated to keyhole limpet haemocyanin.
(Peptide available asab46898)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab43152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanApplication Abreviews Notes WB (1) Use at an assay dependent concentration. Predicted molecular weight: 98 kDa.Notes WB
Use at an assay dependent concentration. Predicted molecular weight: 98 kDa.Target
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Relevance
PML protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. Retinoic acid, a metabolite of vitamin A, is necessary for normal organogenesis but acts as a teratogen at high levels during embryonic and fetal development. Retinoic acid functions through its interaction with the nuclear protein, retinoic acid receptor (RAR). RAR belongs to the steroid and thyroid hormone superfamily of nuclear receptor proteins which exert their effects by binding to specific DNA response elements, thus regulating gene expression in target cells. RAR exists as three major subtypes: alpha, beta and gamma. -
Cellular localization
Nuclear and Cytoplasmic -
Database links
- Entrez Gene: 5371 Human
- Entrez Gene: 5914 Human
- Omim: 102578 Human
- Omim: 180240 Human
- SwissProt: P10276 Human
- SwissProt: P29590 Human
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Alternative names
- MYL antibody
- NR1B1 antibody
- Nuclear receptor subfamily 1 group B member 1 antibody
see all
Images
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Western blot - Anti-PML+RARA Fusion antibody (ab43152)Image courtesy of Christian Marx (University of Jena, Germany)
Anti-PML+ RARα Fusion antibody (ab43152) reactivity with reduced NB4 (A and B) and K562 (B) cell lysate. After SDS-PAGE (where 100μg lysate was loaded in A and 20μg lysate in B), membranes were blocked in 5% Milk in PBS + 0.1% Tween for 1h at 25°C before incubation with ab43152 (1:5,000 dilution in 1% Milk PBS + 0.1% Tween) for 16h at 4ºC. Blots was then incubated with an anti-Rabbit HRP-conjugated secondary antibody before developing with ECL.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (8)
ab43152 has been referenced in 8 publications.
- Kumar S & Tchounwou PB Trisenox Disrupts MDM2-DAXX-HAUSP Complex and Induces Apoptosis in a Mouse Model of Acute Leukemia. J Cancer 11:4373-4383 (2020). PubMed: 32489456
- Rejlova K et al. Low HOX gene expression in PML-RARa-positive leukemia results from suppressed histone demethylation. Epigenetics 13:73-84 (2018). PubMed: 29224413
- Kumar S et al. Trisenox disrupts MDM2-DAXX-HAUSP complex and activates p53, cell cycle regulation and apoptosis in acute leukemia cells. Oncotarget 9:33138-33148 (2018). PubMed: 30237857
- Li K et al. TRIB3 Promotes APL Progression through Stabilization of the Oncoprotein PML-RARa and Inhibition of p53-Mediated Senescence. Cancer Cell 31:697-710.e7 (2017). PubMed: 28486108
- Li X et al. Chemosensitivity enhancement toward arsenic trioxide by inhibition of histone deacetylase in NB4 cell line. J Int Med Res 44:882-92 (2016). PubMed: 27189198
- Hennig D et al. Antagonism between granulocytic maturation and deacetylase inhibitor-induced apoptosis in acute promyelocytic leukaemia cells. Br J Cancer 112:329-37 (2015). WB . PubMed: 25514379
- Ying M et al. Bortezomib sensitizes human acute myeloid leukemia cells to all-trans-retinoic acid-induced differentiation by modifying the RARa/STAT1 axis. Mol Cancer Ther 12:195-206 (2013). WB ; Human . PubMed: 23243061
- Guo Y et al. Blockade of the Ubiquitin Protease UBP43 Destabilizes Transcription Factor PML/RAR{alpha} and Inhibits the Growth of Acute Promyelocytic Leukemia. Cancer Res 70:9875-85 (2010). WB ; Human . PubMed: 20935222
Images
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Western blot - Anti-PML+RARA Fusion antibody (ab43152) Image courtesy of Christian Marx (University of Jena, Germany)
Anti-PML+ RARα Fusion antibody (ab43152) reactivity with reduced NB4 (A and B) and K562 (B) cell lysate. After SDS-PAGE (where 100μg lysate was loaded in A and 20μg lysate in B), membranes were blocked in 5% Milk in PBS + 0.1% Tween for 1h at 25°C before incubation with ab43152 (1:5,000 dilution in 1% Milk PBS + 0.1% Tween) for 16h at 4ºC. Blots was then incubated with an anti-Rabbit HRP-conjugated secondary antibody before developing with ECL.