Anti-Cyclin H/p34 antibody [EPR3929] (ab92376)
![Anti-Cyclin H/p34 antibody [EPR3929] (ab92376)](https://www.abcam.com/ps/products/92/ab92376/Images/ab92376-107350-anti-cyclin-h-antibody-epr3929-western-blot.jpg "Anti-Cyclin H/p34 antibody [EPR3929] (ab92376)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3929] to Cyclin H/p34
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-Cyclin H/p34 antibody [EPR3929]
See all Cyclin H/p34 primary antibodies -
Description
Rabbit monoclonal [EPR3929] to Cyclin H/p34 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human Cyclin H/p34 aa 300-400. The exact sequence is proprietary.
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Positive control
- Jurkat, A431, K562 and HeLa cell lysates, Human breast carcinoma tissue, HeLa cells.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product was previously labelled as Cyclin H
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3929 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cyclin H/p34 antibody [EPR3929] (ab92376)All lanes : Anti-Cyclin H/p34 antibody [EPR3929] (ab92376)
Lane 1 : Jurkat cell lysate
Lane 2 : A431 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 38 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin H/p34 antibody [EPR3929] (ab92376)](https://www.abcam.com/ps/products/92/ab92376/Images/ab92376-107349-anti-cyclin-h-antibody-epr3929-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin H/p34 antibody [EPR3929] (ab92376)ab92376 staining Cyclin H/p34 in Human breast carcinoma tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. (Detection: DAB staining)
Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Cyclin H/p34 antibody [EPR3929] (ab92376)](https://www.abcam.com/ps/products/92/ab92376/Images/ab92376-107348-anti-cyclin-h-antibody-epr3929-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cyclin H/p34 antibody [EPR3929] (ab92376)ab92376 staining Cyclin H/p34 in HeLa cells by Immunofluorescence.
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![Anti-Cyclin H/p34 antibody [EPR3929] (ab92376)](https://www.abcam.com/ps/products/92/ab92376/Images/ab92376-4-benefits-of-recombinant-antibodies.png)
Anti-Cyclin H/p34 antibody [EPR3929] (ab92376)
