Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cyclin A2 antibody [E399] - BSA and Azide free (ab247261)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E399] to Cyclin A2 - BSA and Azide free
  • Suitable for: ICC, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Cyclin A2 antibody [E399] - BSA and Azide free
    See all Cyclin A2 primary antibodies

  • Description

    Rabbit monoclonal [E399] to Cyclin A2 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody recognises Cyclin A2.

  • Tested applications

    Suitable for: ICC, WB, IPmore details
    Unsuitable for: Flow Cyt or IHC

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Epitope

    ab247261 reacts with an epitope located in the N terminal region of Cyclin A2

  • Positive control

    • IP: K-562 whole cell lysate.

  • General notes

    ab247261 is the carrier-free version of ab32498 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247261 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Cyclin A2 antibody [E399] - BSA and Azide free (ab247261)
    Western blot - Anti-Cyclin A2 antibody [E399] - BSA and Azide free (ab247261)
    All lanes : Anti-Cyclin A2 antibody [E399] (ab32498) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : K562 whole cell lysate
    Lane 3 : Jurkat whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 48 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab32498, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The lower band is unidentified.

  • Immunoprecipitation - Anti-Cyclin A2 antibody [E399] - BSA and Azide free (ab247261)
    Immunoprecipitation - Anti-Cyclin A2 antibody [E399] - BSA and Azide free (ab247261)

    This data was developed using ab32498, the same antibody clone in a different buffer formulation.
    Purified ab32498 at 1/20 dilution (1µg) immunoprecipitating Cyclin A2 in K-562 whole cell lysate.
    Lane 1 (input): K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10µg
    Lane 2 (+): ab32498 + K-562 whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32498 in K-562 whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 50 kDa

  • Immunocytochemistry - Anti-Cyclin A2 antibody [E399] - BSA and Azide free (ab247261)
    Immunocytochemistry - Anti-Cyclin A2 antibody [E399] - BSA and Azide free (ab247261)
    This data was developed using ab32498, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of cyclin A expression in HeLa cells using 1/250 ab32498.
  • Anti-Cyclin A2 antibody [E399] - BSA and Azide free (ab247261)
    Anti-Cyclin A2 antibody [E399] - BSA and Azide free (ab247261)

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