Human Cyclin D2 ELISA kit (ab226894)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 23 pg/ml
- Range: 50 pg/ml - 3200 pg/ml
- Sample type: Cell culture extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Cyclin D2 ELISA kit
See all Cyclin D2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% HEK-293T ext 5 2.7% Inter-assay Sample n Mean SD CV% HEK-293T ext 3 6.9% -
Sample type
Cell culture extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
23 pg/ml -
Range
50 pg/ml - 3200 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 92 86% - 97% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Cyclin D2 ELISA kit (ab226894) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cyclin D2 protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Cyclin D2 with 23 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
G1/S-Specific cyclin D2, or Cyclin D2, belongs to the conserved cyclin family of proteins, which have a periodicity in expression levels during the cell cycle and is encoded by the CCND2 gene. Cyclin D2 is a regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family, including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes, which are responsible for the progression through the G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml 10X Human Cyclin D2 Capture Antibody 1 x 600µl 10X Human Cyclin D2 Detector Antibody 1 x 600µl Human Cyclin D2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. -
Sequence similarities
Belongs to the cyclin family. Cyclin D subfamily.
Contains 1 cyclin N-terminal domain. -
Cellular localization
Nucleus. Cytoplasm. Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members. - Information by UniProt
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Alternative names
- CCND 2
- ccnd2
- CCND2_HUMAN
see all -
Database links
- Entrez Gene: 894 Human
- Omim: 123833 Human
- SwissProt: P30279 Human
- Unigene: 376071 Human
Images
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Other - Human Cyclin D2 ELISA kit (ab226894)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of Human Cyclin D2 standard curve in 1X Cell Extraction Buffer PTRBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Cyclin D2 in Human HEK-293T and HL-60 cell extract samplesInterpolated concentrations of native Cyclin D2 in Human HEK-293T cell extract samples based on a 1,250 µg/ml extract load, and interpolated concentrations of spiked Cyclin D2 into HL-60 Cell Extract samples at an extract load of 500 µg/ml. The concentrations of Cyclin D2 were measured in duplicates, interpolated from the Cyclin D2 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cyclin D2 concentration was determined to be 2,612 pg/mL in HEK-293T Cell Extract.
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Sandwich ELISA - Human Cyclin D2 ELISA kit (ab226894)To learn more about the advantages of recombinant antibodies see here.