Antibodies, Proteins, Kits and Reagents for Life Science

318 288 ₸ Product size

100 µl 318 288 ₸ 10 µl 130 665 ₸

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Anti-Cardiac Troponin T antibody [EPR3695] (ab91605)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3695] to Cardiac Troponin T
Suitable for: WB, IP, IHC-P
Reacts with: Human

Product name

Anti-Cardiac Troponin T antibody [EPR3695]
See all Cardiac Troponin T primary antibodies
Description

Rabbit monoclonal [EPR3695] to Cardiac Troponin T
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Cardiac Troponin T aa 50-150. The exact sequence is proprietary.
Positive control
- WB: Human cardiac tissue lysate. IHC-P: Human cardiac muscle tissue. IP: Human heart lysate.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR3695
Isotype

IgG
Research areas
- Cardiovascular
- Cardiovascular Markers

Western blot - Anti-Cardiac Troponin T antibody [EPR3695] (ab91605)

All lanes : Anti-Cardiac Troponin T antibody [EPR3695] (ab91605) at 1/50000 dilution

Lane 1 : Human heart tissue lysate
Lane 2 : Human skeletal muscle tissue lysate
Lane 3 : 293T whole cell lysate

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 36 kDa
Observed band size: 39 kDa
why is the actual band size different from the predicted?

Exposure time: 1 second

Blocking and dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR3695] (ab91605)

ab91605 at 1/100 dilution, staining Cardiac Troponin T in paraffin embedded Human cardiac muscle tissue

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunoprecipitation - Anti-Cardiac Troponin T antibody [EPR3695] (ab91605)

Purified ab91605 at 1/50 dilution (2µg) immunoprecipitating Cardiac Troponin T in Human heart lysate.
Lane 1 (input): Human heart lysate 10µg
Lane 2 (+): ab91605 + Human heart lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab91605 in Human heart lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 39 kDa
Anti-Cardiac Troponin T antibody [EPR3695] (ab91605)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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