Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Syntenin antibody [EPR8102] - BSA and Azide free (ab236071)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8102] to Syntenin - BSA and Azide free
  • Suitable for: IHC-P, IP, ICC/IF, Flow Cyt, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Syntenin antibody [EPR8102] - BSA and Azide free
    See all Syntenin primary antibodies

  • Description

    Rabbit monoclonal [EPR8102] to Syntenin - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HAP1, A549 and HeLa cell lysates.

  • General notes

    ab236071 is the carrier-free version of ab133267 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab236071 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-Syntenin antibody [EPR8102] - BSA and Azide free (ab236071)
    Western blot - Anti-Syntenin antibody [EPR8102] - BSA and Azide free (ab236071)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: Syntenin knockout HAP1 whole cell lysate (20 µg)
    Lane 3: A549 whole cell lysate (20 µg)
    Lane 4: HeLa whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab133267 observed at 32 kDa. Red - loading control, ab130007, observed at 130 kDa.

    ab133267 was shown to recognize Syntenin in wild-type HAP1 cells as signal was lost at the expected MW in Syntenin knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Syntenin knockout samples were subjected to SDS-PAGE. ab133267 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133267).

  • Immunoprecipitation - Anti-Syntenin antibody [EPR8102] - BSA and Azide free (ab236071)
    Immunoprecipitation - Anti-Syntenin antibody [EPR8102] - BSA and Azide free (ab236071)

    ab133267 (purified) at 1/40 immunoprecipitating Syntenin in HeLa whole cell lysate.

    Lane 1 (input): HeLa whole cell lysate (10µg)

    Lane 2 (+): ab133267 + HeLa whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab133267 in HeLa whole cell lysate.

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

     

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133267).

  • Flow Cytometry - Anti-Syntenin antibody [EPR8102] - BSA and Azide free (ab236071)
    Flow Cytometry - Anti-Syntenin antibody [EPR8102] - BSA and Azide free (ab236071)

    Flow Cytometry analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling Syntenin with purified ab133267 at 1/50 (red). Cells were fixed with 4% paraformaldehyde. A goat anti rabbit IgG (Alexa Fluor® 488) 1/2000 was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133267).

  • Immunocytochemistry/ Immunofluorescence - Anti-Syntenin antibody [EPR8102] - BSA and Azide free (ab236071)
    Immunocytochemistry/ Immunofluorescence - Anti-Syntenin antibody [EPR8102] - BSA and Azide free (ab236071)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Syntenin with purified ab133267 at 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

    Negative control 1: PBS only.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133267).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntenin antibody [EPR8102] - BSA and Azide free (ab236071)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntenin antibody [EPR8102] - BSA and Azide free (ab236071)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocellular carcinoma tissue labeling Syntenin with purified ab133267 at 1/50 dilution. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133267).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntenin antibody [EPR8102] - BSA and Azide free (ab236071)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntenin antibody [EPR8102] - BSA and Azide free (ab236071)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cerebral cortex tissue labeling Syntenin with purified ab133267 at 1/50 dilution. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133267).

  • Immunocytochemistry/ Immunofluorescence - Anti-Syntenin antibody [EPR8102] - BSA and Azide free (ab236071)
    Immunocytochemistry/ Immunofluorescence - Anti-Syntenin antibody [EPR8102] - BSA and Azide free (ab236071)

    ab133267 staining Syntenin in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

    Negative control 1: PBS only.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133267).

  • Flow Cytometry - Anti-Syntenin antibody [EPR8102] - BSA and Azide free (ab236071)
    Flow Cytometry - Anti-Syntenin antibody [EPR8102] - BSA and Azide free (ab236071)

    Overlay histogram showing SHSY-5Y cells stained with unpurified ab133267 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133267, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133267).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntenin antibody [EPR8102] - BSA and Azide free (ab236071)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntenin antibody [EPR8102] - BSA and Azide free (ab236071)

    Immunohistochemical analysis of Syntenin in paraffin embedded Human brain tissue, using unpurified ab133267 at a 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133267).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-Syntenin antibody [EPR8102] - BSA and Azide free (ab236071)
    OI-RD Scanning - Anti-Syntenin antibody [EPR8102] - BSA and Azide free (ab236071)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133267).

  • Anti-Syntenin antibody [EPR8102] - BSA and Azide free (ab236071)
    Anti-Syntenin antibody [EPR8102] - BSA and Azide free (ab236071)

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