Anti-Cardiac Troponin T antibody [EPR3696] (ab92546)
![Anti-Cardiac Troponin T antibody [EPR3696] (ab92546)](https://www.abcam.com/ps/products/92/ab92546/Images/ab92546-203530-anti-cardiac-troponin-t-antibody-epr3696-immunohistochemistry.jpg "Anti-Cardiac Troponin T antibody [EPR3696] (ab92546)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3696] to Cardiac Troponin T
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Cardiac Troponin T antibody [EPR3696]
See all Cardiac Troponin T primary antibodies -
Description
Rabbit monoclonal [EPR3696] to Cardiac Troponin T -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human Cardiac Troponin T aa 250-350. The exact sequence is proprietary.
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Positive control
- Human heart lysate; Human skeletal muscle lysate; Mouse heart lysate and Rat heart lysates This antibody gave a positive result in IHC in the following FFPE tissue: Human Heart.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3696 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR3696] (ab92546)
IHC image of Cardiac Troponin T staining in Human Heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab92546, 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Western blot - Anti-Cardiac Troponin T antibody [EPR3696] (ab92546)](https://www.abcam.com/ps/products/92/ab92546/Images/ab92546-107683-anti-cardiac-troponin-t-antibody-epr3696-western-blot.jpg)
Western blot - Anti-Cardiac Troponin T antibody [EPR3696] (ab92546)All lanes : Anti-Cardiac Troponin T antibody [EPR3696] (ab92546) at 1/20000 dilution
Lane 1 : Human heart lysate
Lane 2 : Human skeletal muscle lysate
Lane 3 : Mouse heart lysate
Lane 4 : Rat heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 36 kDa
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![Anti-Cardiac Troponin T antibody [EPR3696] (ab92546)](https://www.abcam.com/ps/products/92/ab92546/Images/ab92546-3-benefits-of-recombinant-antibodies.png)
Anti-Cardiac Troponin T antibody [EPR3696] (ab92546)
