Anti-Cardiac Troponin T antibody [1F11] (ab10214)
![Anti-Cardiac Troponin T antibody [1F11] (ab10214)](https://www.abcam.com/ps/products/10/ab10214/Images/ab10214-6-anti-cardiac-troponin-t-immunocytochemistry-antibody-ips-myocytes-human-1f11.jpg "Anti-Cardiac Troponin T antibody [1F11] (ab10214)")
Key features and details
- Mouse monoclonal [1F11] to Cardiac Troponin T
- Suitable for: Sandwich ELISA, ICC
- Reacts with: Rabbit, Recombinant fragment
- Isotype: IgG2b
Overview
-
Product name
Anti-Cardiac Troponin T antibody [1F11]
See all Cardiac Troponin T primary antibodies -
Description
Mouse monoclonal [1F11] to Cardiac Troponin T -
Host species
Mouse -
Specificity
Suitable for use in Surface Plasmon Resonance Biosensing (PubMed: 20633583 and 20694813) -
Tested applications
Suitable for: Sandwich ELISA, ICCmore details -
Species reactivity
Reacts with: Rabbit, Recombinant fragment -
Immunogen
Free human cardiac troponin T
-
Epitope
Peptide 146-160. -
Positive control
- ICC: ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612)
-
General notes
This product was changed from ascites to tissue culture supernatant on 30th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Concentration varies from lot to lot and can be provided on request.
Does not cross-react with skeletal muscle troponin T, cTnI or TnC.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purity tested by electrophoresis. -
Primary antibody notes
Does not cross-react with skeletal muscle troponin T, cTnI or TnC. -
Clonality
Monoclonal -
Clone number
1F11 -
Myeloma
Sp2/0 -
Isotype
IgG2b -
Research areas
Images
-
Immunocytochemistry - Anti-Cardiac Troponin T antibody [1F11] (ab10214)
Immunofluorescence staining of Cardiac Troponin T using ab10214 in ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612), which were differentiated for 10 days post induction.
The cells were fixed with 100% MeOH (5 min) and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab10214 at 1 µg/mL and ab6046, rabbit polyclonal to beta Tubulin, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150088, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown. Gamma is adjusted to 1.5 in all channels.
The antibody ab10214 also gave a positive staining using 4% formaldehyde fixation (10 min).
-
![Sandwich ELISA - Anti-Cardiac Troponin T antibody [1F11] (ab10214)](https://www.abcam.com/ps/products/10/ab10214/Images/ab10214-17783-anti-cardiac-troponin-t-antibody-1f11-sandwich-elisa.jpg)
Sandwich ELISA - Anti-Cardiac Troponin T antibody [1F11] (ab10214)Standard Curve for Cardiac Troponin T (Analyte: Cardiac Troponin T protein (His tag) (ab86685)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [1F11] to Cardiac Troponin T (ab10214) at 5ug/ml and Detector Antibody Rabbit polyclonal to cardiac Troponin T (ab45932) at 0.5ug/ml.
