Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] - BSA and Azide free (ab250082)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16673(2)] to PPAR alpha + PPAR beta/delta - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] - BSA and Azide free
    See all PPAR alpha + PPAR beta/delta primary antibodies

  • Description

    Rabbit monoclonal [EPR16673(2)] to PPAR alpha + PPAR beta/delta - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250082 is the carrier-free version of ab178865. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250082 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] - BSA and Azide free (ab250082)
    Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] - BSA and Azide free (ab250082)
    All lanes : Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865) at 1/10000 dilution

    Lane 1 : Human PPAR alpha full length recombinant protein
    Lane 2 : Human PPAR beta full length recombinant protein
    Lane 3 : Human PPAR gamma fragment recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 52, 50 kDa



    This data was developed using ab178865, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 10 seconds; Lane 2: 1 second; Lane 3: 3 minutes.

    Human PPAR alpha full length recombinant protein contains aa1-468 with a His-Tag; Human PPAR beta full length recombinant protein contains aa1-441 with a His-Tag; Human PPAR gamma fragment recombinant protein contains aa1-200 with a His-Tag. These three recombinant proteins were made in-house.

  • Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] - BSA and Azide free (ab250082)
    Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] - BSA and Azide free (ab250082)
    All lanes : Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865) at 1/2000 dilution

    Lane 1 : Human fetal liver tissue lysate
    Lane 2 : Human fetal heart tissue lysate
    Lane 3 : Human fetal kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 52, 50 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?


    Exposure time: 2 seconds


    This data was developed using ab178865, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] - BSA and Azide free (ab250082)
    Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] - BSA and Azide free (ab250082)
    All lanes : Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865) at 1/2000 dilution

    Lane 1 : Human skeletal muscle lysate
    Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 5 : Mouse placenta lysate
    Lane 6 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 7 : Rat placenta lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 52, 50 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?



    This data was developed using ab178865, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lanes 1-5: 2 seconds; Lane 6: 1 second; Lane 7: 30 seconds.

  • Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] - BSA and Azide free (ab250082)
    Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] - BSA and Azide free (ab250082)
    All lanes : Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865) at 1/2000 dilution

    Lane 1 : Mouse brain lysate at 10 µg
    Lane 2 : Mouse heart lysate at 10 µg
    Lane 3 : Mouse kidney lysate at 10 µg
    Lane 4 : Mouse spleen lysate at 10 µg
    Lane 5 : Rat brain lysate at 10 µg
    Lane 6 : Rat heart lysate at 10 µg
    Lane 7 : Rat kidney lysate at 10 µg
    Lane 8 : C6 (Rat glial tumor cell line) whole cell lysate at 20 µg
    Lane 9 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
    Lane 10 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg
    Lane 11 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 52, 50 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?



    This data was developed using ab178865, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lanes 1-4: 2 seconds; Lanes 5-11: 1 second.

  • Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] - BSA and Azide free (ab250082)
    Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] - BSA and Azide free (ab250082)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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