Anti-Cardiac Troponin T antibody (ab45932)
Key features and details
- Rabbit polyclonal to Cardiac Troponin T
- Suitable for: IHC-P, Sandwich ELISA, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cardiac Troponin T antibody
See all Cardiac Troponin T primary antibodies -
Description
Rabbit polyclonal to Cardiac Troponin T -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Sandwich ELISA, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Rabbit, Guinea pig, Cow, Cat, Dog
Does not react with:
Mouse -
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human cardiac Troponin T.
Read Abcam's proprietary immunogen policy (Peptide available as ab47005.) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Cardiac Troponin T antibody (ab45932) This image is courtesy of an Abreview submitted by Tova Volberg
ab45932 staining Cardiac Troponin T in rat cardiac cells by ICC/IF (Immunocytochemistry/immunofluorescence).
Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in 3% paraformaldehyde. Samples were incubated with primary antibody (1/400 in diluent) for 1 hour at room temperature. A Cy3®-conjugated goat anti-rabbit polyclonal IgG (1/300) was used as the secondary antibody. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody (ab45932)IHC image of Cardiac Troponin T staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45932, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. -
Western blot - Anti-Cardiac Troponin T antibody (ab45932)Anti-Cardiac Troponin T antibody (ab45932) at 1 µg/ml + Heart (Human) Whole Cell Lysate - fetal normal tissue at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
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Sandwich ELISA - Anti-Cardiac Troponin T antibody (ab45932)Standard Curve for Cardiac Troponin T (Analyte: Cardiac Troponin T protein (His tag) (ab86685)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [1F11] to Cardiac Troponin T (ab10214) at 5ug/ml and Detector Antibody Rabbit polyclonal to cardiac Troponin T (ab45932) at 0.5ug/ml.
