Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cardiac Troponin T antibody [EPR3695] - BSA and Azide free (ab247526)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3695] to Cardiac Troponin T - BSA and Azide free
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Cardiac Troponin T antibody [EPR3695] - BSA and Azide free
    See all Cardiac Troponin T primary antibodies

  • Description

    Rabbit monoclonal [EPR3695] to Cardiac Troponin T - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or ICC

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: Human heart lysate.

  • General notes

    ab247526 is the carrier-free version of ab91605 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247526 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Cardiac Troponin T antibody [EPR3695] - BSA and Azide free (ab247526)
    Western blot - Anti-Cardiac Troponin T antibody [EPR3695] - BSA and Azide free (ab247526)
    All lanes : Anti-Cardiac Troponin T antibody [EPR3695] (ab91605) at 1/50000 dilution

    Lane 1 : Human heart tissue lysate
    Lane 2 : Human skeletal muscle tissue lysate
    Lane 3 : 293T (Human embryonic kidney epithelial cell) whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 36 kDa
    Observed band size: 39 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 second


    This data was developed using ab91605, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR3695] - BSA and Azide free (ab247526)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR3695] - BSA and Azide free (ab247526)
    This data was developed using ab91605, the same antibody clone in a different buffer formulation.ab91605 at 1/100 dilution, staining Cardiac Troponin T in paraffin embedded Human cardiac muscle tissue Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-Cardiac Troponin T antibody [EPR3695] - BSA and Azide free (ab247526)
    Immunoprecipitation - Anti-Cardiac Troponin T antibody [EPR3695] - BSA and Azide free (ab247526)

    This data was developed using ab91605, the same antibody clone in a different buffer formulation.
    Purified ab91605 at 1/50 dilution (2µg) immunoprecipitating Cardiac Troponin T in Human heart lysate.
    Lane 1 (input): Human heart lysate 10µg
    Lane 2 (+): ab91605 + Human heart lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab91605 in Human heart lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 39 kDa

  • Anti-Cardiac Troponin T antibody [EPR3695] - BSA and Azide free (ab247526)
    Anti-Cardiac Troponin T antibody [EPR3695] - BSA and Azide free (ab247526)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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