Anti-Cardiac Troponin I antibody (ab47003)
Key features and details
- Rabbit polyclonal to Cardiac Troponin I
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Cardiac Troponin I antibody
See all Cardiac Troponin I primary antibodies -
Description
Rabbit polyclonal to Cardiac Troponin I -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB MouseHuman
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Immunogen
Synthetic peptide corresponding to Human Cardiac Troponin I aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab47002) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Images
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Western blot - Anti-Cardiac Troponin I antibody (ab47003)All lanes : Anti-Cardiac Troponin I antibody (ab47003) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody (ab47003)IHC image of Cardiac Troponin I staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47003, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. -
Western blot - Anti-Cardiac Troponin I antibody (ab47003)All lanes : Anti-Cardiac Troponin I antibody (ab47003) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Heart (Mouse) Tissue Lysate
Lane 3 : Heart (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Additional bands at: 160 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
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Western blot - Anti-Cardiac Troponin I antibody (ab47003) This image is courtesy of an Abreview submitted by Dr. Mario TorradoAll lanes : Anti-Cardiac Troponin I antibody (ab47003) at 1/20000 dilution
All lanes : Pig Heart whole cell lysate
Lysates/proteins at 4 µg per lane.
Secondary
All lanes : An HRP-conjugated Goat anti-rabbit polyclonal at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted?
Blocking Step: 20% Serum for 3 hours at 24°C -
Immunocytochemistry/ Immunofluorescence - Anti-Cardiac Troponin I antibody (ab47003) This image is courtesy of an anonymous Abreviewab47003 staining Cardiac Troponin I in Human cardiomyocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 30 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS + 1% BSA) for 1 hour at 20°C. An undiluted FITC-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
