Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20307] to Cardiac Troponin I
  • Suitable for: WB, IHC-P, IP, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Cardiac Troponin I antibody [EPR20307]
    See all Cardiac Troponin I primary antibodies

  • Description

    Rabbit monoclonal [EPR20307] to Cardiac Troponin I

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    Rat
    IHC-P
    Mouse
    Rat
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human heart and myocardium lysates; Mouse and rat heart lysates. IHC-P: Human, mouse and rat cardiac muscle tissues. IP: Human fetal heart lysate. IHC-Fr: Mouse heart tissue, Rat heart tissue

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)
    Western blot - Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)
    All lanes : Anti-Cardiac Troponin I antibody [EPR20307] (ab209809) at 1/2500 dilution

    Lane 1 : Human heart lysate
    Lane 2 : Human myocardium lysate
    Lane 3 : Human skeletal muscle lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 24 kDa
    Observed band size: 28 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression of cardiac Troponin I is restricted to cardiac muscle.

    Negative control:

    Human Skeletal muscle

    PMID: 1934363

    PMID: 8661099

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)

    Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Cardiac Troponin I with ab209809 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on human cardiomyocytes [PMID: 22828728]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)
    Immunohistochemistry (Frozen sections) - Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)

    Immunohistochemistry (Frozen) analysis of rat heart tissue section labeling Cardiac Troponin I with purified ab209809 at 1/10 dilution (13.5 µg/ml). Sections were fixed in 0.2% Triton X-100 and permeabilized with DAPI. Antigen retrieval was 4% paraformaldehyde. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/500 dilution (4 µg/ml) dilution. Heat mediated antigen retrieval by using Tris-EDTA buffer (pH9.0) (ab94681) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Frozen sections) - Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)
    Immunohistochemistry (Frozen sections) - Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)

    Immunohistochemistry (Frozen) analysis of mouse heart tissue section labeling Cardiac Troponin I with purified ab209809 at 1/10 dilution (13.5 µg/ml). Sections were fixed in 0.2% Triton X-100 and permeabilized with DAPI. Antigen retrieval was 4% paraformaldehyde. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/500 dilution (4 µg/ml) dilution. Heat mediated antigen retrieval by using Tris-EDTA buffer (pH9.0) (ab94681) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)
    Western blot - Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)
    Anti-Cardiac Troponin I antibody [EPR20307] (ab209809) at 1/5000 dilution + Rat heart lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 24 kDa
    Observed band size: 28 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)
    Western blot - Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)
    All lanes : Anti-Cardiac Troponin I antibody [EPR20307] (ab209809) at 1/2500 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : C2C12 (Mouse myoblast cell line) whole cell lysate
    Lane 3 : Mouse muscle lysate
    Lane 4 : Rat muscle lysate
    Lane 5 : Human brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 24 kDa
    Observed band size: 28 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression of cardiac Troponin I is restricted to cardiac muscle.

    Negative control:

    C2C12, mouse muscle, rat muscle, human brain  

    PMID: 1934363

    PMID: 8661099

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Cardiac Troponin I with ab209809 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: No staining on human skeletal muscle [PMID: 22828728].

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)

    Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Cardiac Troponin I with ab209809 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on mouse cardiomyocytes [PMID: 22828728]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Cardiac Troponin I with ab209809 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: No staining on mouse skeletal muscle [PMID: 22828728].

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)

    Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Cardiac Troponin I with ab209809 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on rat cardiomyocytes [PMID: 22828728]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Cardiac Troponin I with ab209809 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: No staining on rat skeletal muscle [PMID: 22828728].

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)
    Immunoprecipitation - Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)

    Cardiac Troponin I was immunoprecipitated from 0.35 mg of Human fetal heart lysate with ab209809 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab209809 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Human fetal heart lysate, 10 μg (Input).

    Lane 2: ab209809 IP in Human fetal heart lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209809 in Human fetal heart lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)
    Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)

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