Anti-Cardiac Troponin I antibody [4C2] (ab10231)
![Anti-Cardiac Troponin I antibody [4C2] (ab10231)](https://www.abcam.com/ps/products/10/ab10231/Images/ab10231-17831-anti-cardiac-troponin-i-antibody-4c2-immunohistochemistry.jpg "Anti-Cardiac Troponin I antibody [4C2] (ab10231)")
Key features and details
- Mouse monoclonal [4C2] to Cardiac Troponin I
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-Cardiac Troponin I antibody [4C2]
See all Cardiac Troponin I primary antibodies -
Description
Mouse monoclonal [4C2] to Cardiac Troponin I -
Host species
Mouse -
Specificity
reacts with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components (in the presence of 5 mM EDTA). Not affected by heparin, phosphorylation, oxidation and troponin complex formation. Does not cross-react with skeletal muscle troponin I. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein (Human).
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Epitope
23-29 aa -
General notes
Concentration varies from lot to lot and can be provided on request.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purity tested by electrophoresis. -
Clonality
Monoclonal -
Clone number
4C2 -
Myeloma
Sp2/0 -
Isotype
IgG2a -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [4C2] (ab10231)Ab10231 staining human heart tissue. Staining is localised to cytoplasm.
Left panel: with primary antibody at 1ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
