Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824)
Key features and details
- Mouse monoclonal [2F12G2] to Troponin I fast skeletal muscle
- Suitable for: WB, ELISA, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-Troponin I fast skeletal muscle antibody [2F12G2]
See all Troponin I fast skeletal muscle primary antibodies -
Description
Mouse monoclonal [2F12G2] to Troponin I fast skeletal muscle -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseICC/IF HumanWB Human -
Immunogen
Purified full-length recombinant Human Troponin I fast skeletal muscle expressed in E. Coli.
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Positive control
- Recombinant Human TNNI2 protein (AA: 1-182), HEK293 and TNNI2 (AA: 1-182)-hIgGFc transfected HEK293 cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
2F12G2 -
Isotype
IgG1 -
Research areas
Images
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Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824) at 1/500 dilution + Recombinant Human TNNI2 protein (AA: 1-182)
Predicted band size: 21 kDa
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All lanes : Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : TNNI2 (AA: 1-182)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 21 kDa
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Example of standard curves obtained using ab126824. Control antigen 100ng (black), antigen 10ng (purple), antigen 50ng (blue), antigen 100ng (red)
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Immunofluorescence analysis of Hela cells using ab126824 at 1/200 (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Flow cytometric analysis of NIH/3T3 cells using ab126824 AT 1/200 (green) and negative control (red).