Human Cardiac Troponin T ELISA Kit (ab223860)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 68.2 pg/ml
- Range: 195.3 pg/ml - 12500 pg/ml
- Sample type: Cell culture media, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Cardiac Troponin T ELISA Kit
See all Cardiac Troponin T kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 4.82% Inter-assay Sample n Mean SD CV% Serum 3 1.96% -
Sample type
Serum, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
68.2 pg/ml -
Range
195.3 pg/ml - 12500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 88 83% - 93% Tissue Extracts 83 76% - 88% Cell culture media 96 94% - 98% Cit plasma 84 80% - 89% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Cardiac Troponin T ELISA Kit (ab223860) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cardiac Troponin T protein in serum, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Cardiac Troponin T with 68.2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Cardiac Troponin T is one of the three tropomyosin binding subunit that makes up the troponin complex with TnI and TnC being the other two subunits. The troponin complex is essential to the contraction of skeletal and heart muscles. Troponin T specifically binds to tropomyosin and helps position it on actin. Studies have shown elevated levels of troponin T after myocardial infarction and significantly associated with the incidence of heart failure and cardiovascular death.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Cardiac Troponin T Capture Antibody 1 x 600µl 10X Human Cardiac Troponin T Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human Cardiac Troponin T Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. -
Tissue specificity
Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart. -
Involvement in disease
Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function. -
Sequence similarities
Belongs to the troponin T family. - Information by UniProt
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Alternative names
- Cardiac muscle troponin T
- Cardiomyopathy dilated 1D (autosomal dominant)
- Cardiomyopathy hypertrophic 2
see all -
Database links
- Entrez Gene: 7139 Human
- Omim: 191045 Human
- SwissProt: P45379 Human
- Unigene: 533613 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Cardiac Troponin T standard curve in Sample Diluent NS.The Cardiac Troponin T standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Cardiac Troponin T in human heart tissue extract based on a 100 µg/mL extract load.The concentrations of Cardiac Troponin T were measured in duplicate and interpolated from the Cardiac Troponin T standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cardiac Troponin T concentration was determined to be 378.8 pg/mL in heart tissue extract
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Sandwich ELISA - Human Cardiac Troponin T ELISA Kit (ab223860)To learn more about the advantages of recombinant antibodies see here.
