HRP Anti-Cardiac Troponin I antibody [MF4] (ab10239)
![HRP Anti-Cardiac Troponin I antibody [MF4] (ab10239)](https://www.abcam.com/ps/products/10/ab10239/Images/ab10239-172915-anti-cardiac-troponin-i-antibody-mf4-hrp-immunohistochemistry.jpg "HRP Anti-Cardiac Troponin I antibody [MF4] (ab10239)")
Key features and details
- HRP Mouse monoclonal [MF4] to Cardiac Troponin I
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG1
Overview
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Product name
HRP Anti-Cardiac Troponin I antibody [MF4]
See all Cardiac Troponin I primary antibodies -
Description
HRP Mouse monoclonal [MF4] to Cardiac Troponin I -
Host species
Mouse -
Conjugation
HRP -
Specificity
This antibody is reacting with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components (In the presence of 5 mM EDTA). It is not affected by heparin, phosphorylation, oxidation and troponin complex formation. This antibody does not cross-react with skeletal muscle troponin I. Using multiple combinations of MAbs antibodies with different epitope specificity as capture and multiple MAbs as detection, the sensitivity of the sandwich ELISA can be increased 2-4 fold. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
free human cardiac troponin and/or native troponin complex.
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Epitope
190-196 aa -
Positive control
- IHC-P: Human heart FFPE tissue sections.
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General notes
Concentration varies from lot to lot and can be provided on request.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.05% Proclin 300
Constituents: 0.8% Sodium chloride, 0.02% Potassium chloride, 0.024% Monobasic dihydrogen potassium phosphate, 0.18% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purity tested by electrophoresis. -
Clonality
Monoclonal -
Clone number
MF4 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Cardiac Troponin I antibody [MF4] (ab10239)
IHC image of Cardiac Troponin I staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10239, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
